{"title":"IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.","authors":"Yasuo Fukutomi, Masanori Matsuoka, Fumishige Minagawa, Satoshi Toratani, Gregory McCormick, James Krahenbuhl","doi":"10.1489/1544-581X(2004)072<0016:ITOMBI>2.0.CO;2","DOIUrl":null,"url":null,"abstract":"<p><p>In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.</p>","PeriodicalId":14078,"journal":{"name":"International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association","volume":"72 1","pages":"16-26"},"PeriodicalIF":0.0000,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1489/1544-581X(2004)072<0016:ITOMBI>2.0.CO;2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
Abstract
In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
