[The analysis of rbcS gene function by post-transcription gene silencing in Nicotiana benthamiana].

Xiao-Fu Zhou, Peng-Da Ma, Ren-Hou Wang, Xiao-Juan Zhu, Bao Liu, Xing-Zhi Wang
{"title":"[The analysis of rbcS gene function by post-transcription gene silencing in Nicotiana benthamiana].","authors":"Xiao-Fu Zhou, Peng-Da Ma, Ren-Hou Wang, Xiao-Juan Zhu, Bao Liu, Xing-Zhi Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A system of virus-induced post-transcriptional gene silencing for studying rbcS gene function was established and optimized using tobacco rattle virus vector and Nicotiana benthamiana as experimental materiaes. The following analyses were conducted: phenotypic characterization of rbcS gene silenced plants, transcription levels of rbcS gene by RT-PCR; protein levels of rbcS by the antibodies of rbcS and rbcL and photosynthetic pigments wntents in rbcS silenced plants by HPLC method. The results showed that the seedlings at 21-24-day-old and Agrobacterium concentration at OD600 = 1-1.5 gave the best results for gene silencing. The expression level of rbcL was very likely regulated by rbcS, and rbcS gene did not relate to the collection of photosynthetic energy. Probability analysis showed that the tobacco rattle virus vector system is a useful and effective technique to study rbcS gene function via post-transcriptional gene silencing.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 6","pages":"586-93"},"PeriodicalIF":0.0000,"publicationDate":"2005-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yi chuan xue bao = Acta genetica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

A system of virus-induced post-transcriptional gene silencing for studying rbcS gene function was established and optimized using tobacco rattle virus vector and Nicotiana benthamiana as experimental materiaes. The following analyses were conducted: phenotypic characterization of rbcS gene silenced plants, transcription levels of rbcS gene by RT-PCR; protein levels of rbcS by the antibodies of rbcS and rbcL and photosynthetic pigments wntents in rbcS silenced plants by HPLC method. The results showed that the seedlings at 21-24-day-old and Agrobacterium concentration at OD600 = 1-1.5 gave the best results for gene silencing. The expression level of rbcL was very likely regulated by rbcS, and rbcS gene did not relate to the collection of photosynthetic energy. Probability analysis showed that the tobacco rattle virus vector system is a useful and effective technique to study rbcS gene function via post-transcriptional gene silencing.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
[通过转录后基因沉默分析烟曲霉的 rbcS 基因功能]。
以烟草鼠疫病毒载体和烟草烟碱为实验材料,建立并优化了病毒诱导转录后基因沉默系统,用于研究rbcS基因的功能。对沉默rbcS基因植株的表型特征、RT-PCR法检测rbcS基因转录水平、rbcS和rbcL抗体检测rbcS蛋白水平、HPLC法检测rbcS沉默植株光合色素含量进行了分析。结果表明,21-24 天的幼苗和农杆菌浓度为 OD600 = 1-1.5 的基因沉默效果最好。rbcL 的表达水平很可能受 rbcS 的调控,而 rbcS 基因与光合作用能量的收集无关。概率分析表明,烟草鼠疫病毒载体系统是通过转录后基因沉默研究 rbcS 基因功能的有效技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[Influence of B mating-type factor on recovery of nuclear types from dikaryons in Lentinula edodes]. [Expression and immunization testing of fusion protein of Newcastle disease virus in leaf tissue of transgenic rice]. Nuclear matrices and matrix attachment regions from Green alga: Dunaliella salina. [Molecular basic of interaction between disease resistance gene and avirulence gene]. [Progress in the study on diacylgycerol acyltransferase (DGAT)-related genes].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1