[Influence of B mating-type factor on recovery of nuclear types from dikaryons in Lentinula edodes].

Shui-Ming Cheng, Fang-Can Lin
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Abstract

This study determined a genetic basis for the phenomenon of significant asymmetric ratio of the two component nuclear types from dikaryons of Lentinula edodes via protoplast formation and regeneration by clarifying the influence of A or B mating-type factor on recovery of nuclear types from dikuaryons. The results revealed that B factor or some supposed genes linked to the B factor appeared to influence survival of dedikaryotized nuclei,whereas A factor had no apparent effect on this phenomenon. Specificity of B factor was shown to correlate with percentage survival of the component nuclear types recovered from both kinds of heterokaryons (A not equal to B not equal to and A = B not equal to). It can be put in an hierarchical order with respect to this function,the hierarchical order of these B factors was B1 > B3 > B4 > B2 between two tested strains.

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[B交配型因子对香菇双核子核型恢复的影响]。
本研究通过阐明a或B交配型因子对双核子核型恢复的影响,确定了香菇双核子在原生质体形成和再生过程中两组分核型比例显著不对称现象的遗传基础。结果表明,B因子或与B因子相关的基因可能影响去双核细胞核的存活,而A因子对这一现象无明显影响。结果表明,B因子的特异性与从两种异核体(A不等于B不等于和A = B不等于)中恢复的组成核类型的存活率相关。B因子在两株间的作用顺序为B1 > B3 > B4 > B2。
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[Influence of B mating-type factor on recovery of nuclear types from dikaryons in Lentinula edodes]. [Expression and immunization testing of fusion protein of Newcastle disease virus in leaf tissue of transgenic rice]. Nuclear matrices and matrix attachment regions from Green alga: Dunaliella salina. [Molecular basic of interaction between disease resistance gene and avirulence gene]. [Progress in the study on diacylgycerol acyltransferase (DGAT)-related genes].
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