[Effects of microsatellite DNA markers on meat quality traits in pig chromosome 13].

Gen-Bao Shao, Chao Jia, Ru-Qian Zhao, Jie Chen, Xiao-Jing Yang, Rui-Hua Huang, Yin-Xue Xu, Hong-Lin Liu
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Abstract

In this reseanch, 7 microsatellite DNA loci linked with PPAR gene were selected from the published genetic map of chromosome 13 in pig,and polymorphisms of these microsatellites in 100 samples from Sutai pigs (Duroc x Erhualian) populations were detected. Results revealed that the number of alleles were 6-9, heterozygosity 0.59 - 0.81, polymorphism information content 0.51 - 0.76. Effects of S0021, SW1937, SW482, S0222, S0293, S0281 and SWR2054 on meat quality traits were analyzed with PROC GLM of SAS. Results showed that the effects of S0021 on pH value and SW937 on water-holding capacity reached a significant level at P < 0.01 respectively. The effect of S0293 on tenderness and SW482 on BFT were also significant (P < 0.05). S0222, S0281 and SWR2054 had no significant effect on the 7 selected meat qualitytraits (P>0.05).

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微卫星DNA标记对猪13号染色体肉质性状的影响
本研究从已发表的猪13号染色体遗传图谱中选择了7个与PPAR基因相关的微卫星DNA位点,并在苏泰猪(杜洛和二华联)群体的100个样品中检测了这些微卫星的多态性。结果表明,共有6 ~ 9个等位基因,杂合度为0.59 ~ 0.81,多态性信息含量为0.51 ~ 0.76。采用SAS PROC GLM分析S0021、SW1937、SW482、S0222、S0293、S0281和SWR2054对肉质性状的影响。结果表明,S0021对土壤pH值的影响达到极显著水平,SW937对土壤持水量的影响达到极显著水平(P < 0.01)。S0293对压痛和SW482对BFT的影响也显著(P < 0.05)。S0222、S0281和SWR2054对所选7项肉质性状无显著影响(P>0.05)。
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