[QTL mapping and interaction analysis of genotype x environment (Fe2+ -concentrations ) for mesocotyl length in rice (Oryza sativa L.)].

You-Nan Ouyang, Qiu-Ying Zhang, Ke-Qin Zhang, Sheng-Miao Yu, Jie-Yun Zhuang, Qian-Yu Jin, Shi-Hua Cheng
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Abstract

A recombinant inbred lines (RILs) population derived from a cross between Zhenshan97B and Miyang46 was used for detecting QTLs with additive effects and additive-by-additive epistasis for rice mesocotyl length. A linkage map consisting of 207 DNA markers,distributing on the 12 chromosomes of rice,was employed for QTL mapping by using software QTL Mapper 1.6 of mixed linear model. Rice mesocotyl length under germination conditions with 4 different FeSO4 concentrations (0, 1.79, 7.16, 14.32 mmol/L) was measured 7 days after planting. A total of 6 QTLs with significant additive effects on chromosome 1, 5 and 9, with variance explained of 3.5%-11.4%, eleven QTLs with significant additive x additive epistatic effects on chromosome 1, 2, 3, 4, 5, 8 were detected, with variance explained of 4.5%-8.1%. In addition, one QTL for environmental interaction (Fe2+ -concentrations) was detected.

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水稻(Oryza sativa L.)中胚轴长度基因型x环境(Fe2+浓度)QTL定位与互作分析[j]。
利用珍汕97b与密阳46杂交的重组自交系(RILs)群体,对水稻中胚轴长度具有加性效应和加性间上位性的qtl进行了检测。利用混合线性模型软件QTL Mapper 1.6对分布在水稻12条染色体上的207个DNA标记进行QTL定位。测定了4种不同浓度FeSO4(0、1.79、7.16、14.32 mmol/L)萌发条件下水稻中胚轴长度。在1、5、9号染色体上共检测到6个显著加性效应的qtl,方差解释范围为3.5% ~ 11.4%;在1、2、3、4、5、8号染色体上共检测到11个显著加性x加性显性效应的qtl,方差解释范围为4.5% ~ 8.1%。此外,还检测到1个环境相互作用QTL (Fe2+ -浓度)。
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