Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe.

IF 1.5 4区医学 Q3 PARASITOLOGY Folia Parasitologica Pub Date : 2021-02-02 DOI:10.14411/fp.2021.002

Martin Kvac, Eva Myskova, Nikola Holubova, Klara Kellnerova, Marta Kicia, Dusan Rajsky, John McEvoy, Yaoyu Feng, Vladimir Hanzal, Bohumil Sak

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引用次数: 7

Abstract

Parasites of the genus Cryptosporidium Tyzzer, 1910 are one of the most common protistan parasites of vertebrates. Faecal samples from 179 red foxes (Vulpes vulpes [Linnaeus]), 100 grey wolves (Canis lupus Linnaeus), 11 golden jackals (Canis aureus Linnaeus), and 63 brown bears (Ursus arctos Linnaeus) were collected in the Czech Republic, Poland and Slovakia. Samples were examined for the presence of Cryptosporidium spp. using microscopy and PCR/sequence analysis. Phylogenetic analysis based on the small subunit ribosomal RNA (SSU), actin and 60-kDa glycoprotein (gp60) genes using the maximum likelihood method revealed the presence of Cryptosporidium tyzzeri Ren, Zhao, Zhang, Ning, Jian et al., 2012 (n = 1) and C. andersoni Lindsay, Upton, Owens, Morgan, Mead et Blackburn, 2000 (n = 2) in red foxes, C. canis Fayer, Trout, Xiao, Morgan, Lai et Dubey, 2001 (n = 2) and C. ubiquitum Fayer, Santín et Macarisin, 2010 (n = 2) in grey wolves, and C. galli Pavlásek, 1999 in brown bears (n = 1) and red foxes (n = 1). Subtyping of isolates of C. ubiquitum and C. tyzzeri based on sequence analysis of gp60 showed that they belong to the XIId and IXa families, respectively. The presence of specific DNA of C. tyzzeri, C. andersoni and C. galli, which primarily infect the prey of carnivores, is probably the result of their passage through the gastrointestinal tract of the carnivores. Finding C. ubiquitum XIId in wolves may mean broadening the host spectrum of this subtype, but it remains possible this is the result of infected prey passing through the wolf - in this case deer, which is a common host of this parasite. The dog genotype of C. canis was reported for the first time in wolves.

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隐孢子虫在中欧野生狐狸、狼、豺狼和熊中的发生和遗传多样性。

隐孢子虫属(Cryptosporidium Tyzzer, 1910)是脊椎动物最常见的原生寄生虫之一。在捷克、波兰和斯洛伐克采集了179只红狐(Vulpes Vulpes [Linnaeus])、100只灰狼(Canis lupus Linnaeus)、11只金豺(Canis aureus Linnaeus)和63只棕熊(Ursus arctos Linnaeus)的粪便样本。使用显微镜和PCR/序列分析检测样品是否存在隐孢子虫。系统发育分析基于小亚基的核糖体RNA(四)、肌动蛋白和60-kDa糖蛋白(gp60)基因使用最大似然方法揭示隐孢子虫的存在tyzzeri任,赵、张、Ning,剑et al ., 2012 (n = 1)和c . andersoni林赛,厄普顿•欧文斯,摩根,米德布莱克本,2000年红狐狸(n = 2), c .犬属律师法,鳟鱼,肖,摩根,赖Dubey, 2001 (n = 2)和c . ubiquitum律师法,Santin Macarisin, 2010年灰狼(n = 2),在棕熊(n = 1)和红狐(n = 1)中发现的C. ubiquitum和C. tyzzeri菌株Pavlásek, 1999。基于gp60序列分析，它们分别属于XIId和IXa科。C. tyzzeri、C. andersoni和C. galli主要感染食肉动物的猎物，它们的特定DNA的存在可能是它们通过食肉动物胃肠道的结果。在狼身上发现C. ubiquitum XIId可能意味着拓宽了这种亚型的宿主范围，但仍然有可能是被感染的猎物通过狼的身体传播的结果——在这种情况下，鹿是这种寄生虫的常见宿主。在狼中首次报道了犬源性犬瘟热基因型。

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来源期刊

Folia Parasitologica 医学-寄生虫学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.