RT-PCR Screening Tests for SARS-CoV-2 with Saliva Samples in Asymptomatic People: Strategy to Maintain Social and Economic Activities while Reducing the Risk of Spreading the Virus.

Abstract

The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35

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无症状人群唾液样本检测SARS-CoV-2的RT-PCR筛查:维持社会经济活动同时降低病毒传播风险的策略

2020年将被人们铭记，因为2019冠状病毒病(COVID-19)大流行继续影响全世界。早期准确识别严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)对抗击该疾病至关重要。在目前的诊断检测中，实时逆转录聚合酶链反应(RT-qPCR)是最可靠、最常用的方法。在这里，我们讨论了RT-qPCR结果与病毒感染性的解释。虽然鼻咽拭子样本经常用于RT-qPCR检测，但它们需要由训练有素的医务人员收集。唾液样本正在成为一种廉价而有效的大规模筛查方法。唾液混合样本检测已被应用于SARS-CoV-2感染的大规模筛查。目前的政策建议隔离周期阈值(Ct)为35的患者

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