Archana Bhartiya, Darren Batey, Silvia Cipiccia, Xiaowen Shi, Christoph Rau, Stanley Botchway, Mohammed Yusuf, Ian K Robinson
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引用次数: 0
Abstract
Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced 'X-ray karyotypes' of both heavy metal-stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.
对染色体在细胞遗传学中的结构和功能作用的研究已经持续了十多年。在这项工作中，我们利用最新同步辐射源的相干 X 射线，使用硬 X 射线层析技术提取了人类 B 细胞和 T 细胞分裂期全部 46 条染色体的个体质量。我们制作了重金属染色和未染色涂片的 "X 射线核型"，以确定低剂量 X 射线辐照造成辐射损伤后遗传物质的增减。实验在英国 Didcot 的钻石光源 I-13 光束线进行，使用的是相位敏感 X 射线层析成像法。
期刊介绍：
Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to:
· Chromosomes and their linkage to diseases;
· Chromosome organization within the nucleus;
· Chromatin biology (transcription, non-coding RNA, etc);
· Chromosome structure, function and mechanics;
· Chromosome and DNA repair;
· Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting,
dosage compensation, sex determination, chromosome remodeling);
· Architectural/epigenomic organization of the genome;
· Functional annotation of the genome;
· Functional and comparative genomics in plants and animals;
· Karyology studies that help resolve difficult taxonomic problems or that provide
clues to fundamental mechanisms of genome and karyotype evolution in plants and animals;
· Mitosis and Meiosis;
· Cancer cytogenomics.
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