Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using ^99mTc-sulfur colloid functional imaging.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Receptors and Signal Transduction Pub Date : 2022-06-01 Epub Date: 2021-04-15 DOI:10.1080/10799893.2021.1912097

Fatma J Al-Saeedi, Salah Kh Al-Waheeb, Peramaiyan Rajendran, Khalid M Khan, Moudhi Sadan

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Abstract

This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (^99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. ^99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.

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99mtc -硫胶体功能显像显示，早期注射胰岛素可减轻链脲佐菌素诱导的糖尿病大鼠肝脏的组织学和功能变化。

本研究旨在探讨胰岛素对糖尿病大鼠肝脏和脾脏网状内皮系统(RES)的影响。将Sprague Dawley大鼠分为对照组、糖尿病大鼠(DM)和胰岛素治疗2周的糖尿病大鼠(IDM)。用锝-99m-硫胶体(99mTc-SC)示踪剂对大鼠成像，通过绘制感兴趣的区域来确定示踪剂在各组的区域分布，然后得到心、肝、脾的累积计数与全身(WB)的比值。取各组大鼠肝组织进行光镜、电镜观察。与对照组和IDM大鼠相比，DM大鼠的mtc - sc摄取比显示肝脏对WB的摄取比较低。电镜显示DM大鼠肝细胞严重空泡化。IDM大鼠表现出完全的空泡化。糖尿病大鼠早期给予胰岛素2周，可明显改善肝脏吞噬功能和组织学改变。

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来源期刊

Journal of Receptors and Signal Transduction 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Receptors and Signal Tranduction is included in the following abstracting and indexing services: BIOBASE; Biochemistry and Biophysics Citation Index; Biological Abstracts; BIOSIS Full Coverage Shared; BIOSIS Previews; Biotechnology Abstracts; Current Contents/Life Sciences; Derwent Chimera; Derwent Drug File; EMBASE; EMBIOLOGY; Journal Citation Reports/ Science Edition; PubMed/MedLine; Science Citation Index; SciSearch; SCOPUS; SIIC.