[Microsatellite marker linked with stripe rust resistant gene Yr9 in wheat].

Dong-Xu Weng, Shi-Chang Xu, Rui-Ming Lin, An-Min Wan, Jing-Peng Li, Li-Ren Wu
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Abstract

SSR analysis was performed using a wheat near-isogenic line (NIL) Taichuang29 * 6/ Lovrin13, which carried the resistance gene Yr9 against wheat stripe rust and its recurrent parent Taichung29 as materials. After screening with 32 SSR primers on 1B chromosome, reproducible polymorphic DNA fragment amplified by Xgwm582 was identified. Genetic linkage was tested in 177 segregating F2 plants. The results indicated that microsatellite marker Xgwm582 was linked with gene Yr9 resistant to wheat stripe rust. A genetic distance of 3. 7 cM was calculated.

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[小麦抗条锈病基因Yr9连锁微卫星标记]。
以携带小麦条锈病抗性基因Yr9的小麦近等基因系Taichuang29 * 6/ Lovrin13及其复发亲本taichun29为材料,进行SSR分析。通过对1B染色体上32条SSR引物的筛选,鉴定出Xgwm582扩增的可复制多态性DNA片段。对177个F2分离植株进行了遗传连锁试验。结果表明,微卫星标记Xgwm582与小麦条锈病抗性基因Yr9连锁。遗传距离为3。计算7cm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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