[Study on early stability by ISSR markers in rice].

Abstract

In the F2 population crossed from two early stability rice (Oryza sativa L.) with four cultivars, eight uniform strains were recorded. Genetic analysis showed that both uniform strains with uniform agronomic characteristics and segregated strains segregating in Mendelian manner were observed in F2 population of the same combination. Four types of marker-bands were obtained after the F2 uniform strains were marked by 26 ISSR primers:t ype I, maternal marker bands present, and paternal marker bands absent; type II, paternal marker bands present, and maternal marker bands absent; type III, parts of maternal and paternal marker bands present, and the others absent; type IV, new recombined marker bands appeared, and all maternal and paternal marker bands absent. But segregating strains showed heterzygosity marker bands, maternal and paternal marker bands being present. The uniform strains and normal strains in rice might be grouped into two classes on the basis of the 2000 bp marker band amplified by the ISSR marker primers No. 900. This result would provide experimental basis for the study on genetic mechanism of early stability in rice.