Volodya Hayrapetyan, Monica Jenschke, Glenn H. Dillon, Tina K. Machu
{"title":"Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity","authors":"Volodya Hayrapetyan, Monica Jenschke, Glenn H. Dillon, Tina K. Machu","doi":"10.1016/j.molbrainres.2005.09.011","DOIUrl":null,"url":null,"abstract":"<div><p>Allosteric modulation of mouse 5-Hydroxytryptamine<sub>3A</sub> (5-HT<sub>3A</sub>) and 5-HT<sub>3A/B</sub><span> receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT</span><sub>3A</sub> receptor function, but had no effect on mouse 5-HT<sub>3A/B</sub> receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT<sub>3A/B</sub> receptor function was much less than that observed in the mouse 5-HT<sub>3A</sub> receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20–80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT<sub>3</sub> receptors.</p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"142 2","pages":"Pages 146-150"},"PeriodicalIF":0.0000,"publicationDate":"2005-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.011","citationCount":"25","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Brain Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0169328X05003724","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 25
Abstract
Allosteric modulation of mouse 5-Hydroxytryptamine3A (5-HT3A) and 5-HT3A/B receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT3A receptor function, but had no effect on mouse 5-HT3A/B receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT3A/B receptor function was much less than that observed in the mouse 5-HT3A receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20–80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT3 receptors.
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