Characterization and expression analysis of a MADS box gene, HoMADS2, in Hyacinthus orientalis L.

Hong-Yan Su, Quan-Zi Li, Xing-Guo Li, Xian-Sheng Zhang
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Abstract

A MADS box gene HoMADS2 was cloned from Hyacinthus orientalis L. in this study. Sequence comparison revealed that HoMADS2 was highly homologous to the class B MADS box genes. Furthermore, phylogenetic analysis showed that HoMADS2 was closely related to PI gene family and this was also supported by the presence of specific diagnostic sites of PI homologs in K box domain and C terminal region,suggesting that HoMADS2 might be a PI-like gene. HoMADS2 mRNA was accumulated in all floral organs,different from the expression patterns of PI homologs in dicots. During in vitro flower development, HoMADS2 expression was constitutively expressed and not affected by the presence of cytokinin and auxin in the regenerated flowers. Our results indicated that the expression of HoMADS2 is different from those of both HAG1 and HoMADS1 in responding to plant hormones during in vitro flower development.

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风信子(Hyacinthus orientalis L.) MADS盒子基因HoMADS2的表达分析。
本研究从风信子中克隆了一个MADS box基因HoMADS2。序列比较表明,HoMADS2与B类MADS box基因高度同源。此外,系统发育分析表明,HoMADS2与PI基因家族关系密切,并且在K盒域和C端区存在PI同源物的特异性诊断位点,表明HoMADS2可能是PI样基因。HoMADS2 mRNA在所有花器官中均有积累,但不同于PI同源物在薯蓣中的表达模式。在离体花发育过程中,HoMADS2的表达是组成型表达,不受细胞分裂素和生长素存在的影响。我们的研究结果表明,在离体花发育过程中,HoMADS2的表达与HAG1和HoMADS1的表达对植物激素的响应是不同的。
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