The effect of RAB5A gene on rearrangement of microfilaments in human lung adenocarcinoma cells.

Abstract

To study the effect of RAB5A gene on microfilaments in human lung adenocarcinoma cells, AGZY83-a cells were stained with fluorescein isothiocyanate (FITC)-phalloidin. Microfilament bundles in RAB5A gene over-expressed AGZY83-a cells were shown to be denser than in control cells using confocal laser scanning microscope (CLSM). According to the analysis of Tumor Metastasis Microarray on RAB5A gene, three genes related to the regulation of cytoskeleton were identified, including NM23H1, Rac1 (downregulated in RAB5A over expressing cells), and S100A4 (upregulated in RAB5A overexpressing cells). Previous studies demonstrated that S100A4 gene functioned to suppress the expression of NM23H1 gene. To test if this elevated expression of S100A4 results in the down-regulation of NM23H1, RNAi was applied to silence the expression of S100A4 in AGZY83-a cells. Our data indicated that expression of NM23H1 was increased following the inhibition of S100A4 expression. Altogether, the results indicated that RAB5A gene was involved in the suppression of expression of NM23H1 by promoting the expression of S100A4.