Truncated Semenogelin I Binds Zinc and Is Cleaved by Prostate-Specific Antigen

Magnus Jonsson, Åke Lundwall, Sara Linse, Birgitta Frohm, Johan Malm
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引用次数: 8

Abstract

ABSTRACT: Semenogelins I and II are major coagulum-forming proteins in semen, and they are secreted mainly by the seminal vesicles. These proteins bind Zn2+ and act as substrates for prostate-specific antigen and transglutaminase. A variant semenogelin I lacking 60 amino acids has been described that occurs in different populations with an allele frequency of 1%–3%. To better understand the function of the semenogelins in vivo, our aim was to characterize the properties of the variant form and compare with the wild type. Recombinant proteins were synthesized in insect cells. Binding of Zn2+ was studied by titration of metal ions in the presence of a zinc (II) fluorophore chelator. SDS-PAGE was used to visualize the results of cleavage by prostate-specific antigen and cross-linking with transglutaminase. We found that the truncated and wild-type semenogelin molecules had similar Zn2+-binding properties (ie, a stoichiometry of at least 9–10 mol per mol of protein and an average dissociation constant of 5 μmol/L per site), and they showed also similar susceptibility for degradation by prostate-specific antigen. Furthermore, like the wild-type form, the truncated semenogelin I was able to serve as a substrate for transglutaminase. These findings imply that the studied characteristics do not depend on a well-defined tertiary structure, or that the deletion has no major effect on the structure responsible for these features.

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截断的Semenogelin I结合锌并被前列腺特异性抗原切割
摘要:精凝胶蛋白I和II是精液中主要的凝血形成蛋白,主要由精囊分泌。这些蛋白结合Zn2+并作为前列腺特异性抗原和谷氨酰胺转酶的底物。一种缺少60个氨基酸的变异体semenogelin I已被描述,它发生在不同的人群中,等位基因频率为1%-3%。为了更好地了解semenogelins在体内的功能,我们的目的是表征变异形式的特性,并与野生型进行比较。在昆虫细胞中合成了重组蛋白。在锌(II)荧光团螯合剂的存在下,用金属离子滴定法研究了Zn2+的结合。SDS-PAGE用于显示前列腺特异性抗原裂解和转谷氨酰胺酶交联的结果。我们发现,截断型和野生型的semenogelin分子具有相似的Zn2+结合特性(即,至少9-10 mol/ mol蛋白质的化学测量和平均解离常数为5 μmol/L每个位点),并且它们对前列腺特异性抗原的降解也表现出相似的敏感性。此外,就像野生型一样,截断的semenogelin I能够作为转谷氨酰胺酶的底物。这些发现意味着所研究的特征并不依赖于一个明确的三级结构,或者缺失对负责这些特征的结构没有主要影响。
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来源期刊
Journal of andrology
Journal of andrology 医学-男科学
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审稿时长
5.6 months
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