[Tumor necrosis factor alpha (TNF-alpha) gene G-308A polymorphism relationship to insulin resistance and lipid abnormalities in children with obesity].

Beata Pyrzak, Alicja Wiśniewska, Barbara Rymkiewicz-Kluczyńska
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Abstract

Introductions: High levels of tumor necrosis factor (TNF-alpha)-cytokine produced by adipocytes, are involved in the development of obesity-related insulin resistance and metabolic syndrome. Therefore, we investigated whether the polymorphism of TNF-alpha-308 gene can predict the conversion from insulin resistance and obesity parameters in children with obesity. THE STUDY comprised 72 children with obesity simplex (9-18 y.o). The following anthropometric parameters: weight, height, BMI, SDS for BMI, WHR, sum of 3, 10 skinfolds and percentage of body fat by Slaughter's equation were calculated. In each child, after 12 hour overnight fast, glucose, insulin, leptin and lipids: triglycerides (Tg), cholesterol total (Chol-T), cholesterol HDL (Chol-HDL), cholesterol LDL (Chol-LDL) were measured. The oral glucose tolerance test was performed and HOMA-IR was calculated.

Results: Using the technique of PCR-RELP and SSCP, three variants of genotype TNF-alpha were obtained: G/G-68%, A/G-29%, A/A-3%. Statistical analysis of anthropometric, biochemical and leptin variables in groups G/G, vs. A/G +A/A, in boys and girls was performed. We did not find any significant differences between groups (G/G vs. A/G +A/A in all group, and between G/G girls, boys vs A/G +A/A girls, boys) Our data indicate that in smaller children studies, TNF-alpha polymorphism does not seem not to be associated with the degree of obesity and insulin resistance.

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[肿瘤坏死因子α (tnf - α)基因G-308A多态性与肥胖儿童胰岛素抵抗和脂质异常的关系]。
高水平的肿瘤坏死因子(tnf - α)-一种由脂肪细胞产生的细胞因子,参与肥胖相关胰岛素抵抗和代谢综合征的发展。因此,我们研究了tnf - α -308基因多态性是否可以预测肥胖儿童胰岛素抵抗和肥胖参数的转换。该研究纳入了72名单纯性肥胖儿童(9-18岁)。计算下列人体测量参数:体重、身高、BMI、BMI的SDS、臀重比、3、10个皮褶的总和和体脂率(采用Slaughter’s方程)。各组患儿禁食12小时后,测定血糖、胰岛素、瘦素及血脂:甘油三酯(Tg)、总胆固醇(cholt)、胆固醇高密度脂蛋白(choll -HDL)、胆固醇低密度脂蛋白(choll -LDL)。进行口服糖耐量试验,计算HOMA-IR。结果:采用PCR-RELP和SSCP技术,获得3个基因型变异:G/G-68%, A/G-29%, A/A-3%。统计分析G/G组、A/G +A/A组男孩和女孩的人体测量学、生化和瘦素变量。我们没有发现组间的任何显著差异(所有组中G/G vs. A/G +A/A,以及G/G女孩、男孩与A/G +A/A女孩、男孩之间)。我们的数据表明,在较小的儿童研究中,tnf - α多态性似乎与肥胖程度和胰岛素抵抗无关。
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