Mechanism of DNA transport through pores.

Abstract

The transport of electrically charged macromolecules such as DNA through narrow pores is a fundamental process in life. When polymer molecules are forced to navigate through pores, their transport is controlled by entropic barriers that accompany their conformational changes. During the past decade, exciting results have emerged from single-molecule electrophysiology experiments. Specifically, the passage of single-stranded DNA/RNA through alpha-hemolysin pores and double-stranded DNA through solid-state nanopores has been investigated. By a combination of these results with the entropic barrier theory of polymer transport and macromolecular simulations, an understanding of the mechanism of DNA transport through pores has emerged.