The roles of hypoxia in the in vitro engineering of tissues.

Jos Malda, Travis J Klein, Zee Upton
{"title":"The roles of hypoxia in the in vitro engineering of tissues.","authors":"Jos Malda,&nbsp;Travis J Klein,&nbsp;Zee Upton","doi":"10.1089/ten.2006.0417","DOIUrl":null,"url":null,"abstract":"<p><p>Oxygen is a potent modulator of cell function and wound repair in vivo. The lack of oxygen (hypoxia) can create a potentially lethal environment and limit cellular respiration and growth or, alternatively, enhance the production of the specific extracellular matrix components and increase angiogenesis through the hypoxia-inducible factor-1 pathway. For the in vitro generation of clinically relevant tissue-engineered grafts, these divergent actions of hypoxia should be addressed. Diffusion through culture medium and tissue typically limits oxygen transport in vitro, leading to hypoxic regions and limiting the viable tissue thickness. Approaches to overcoming the transport limitations include culture with bioreactors, scaffolds with artificial microvasculature, oxygen carriers, and hyperbaric oxygen chambers. As an alternate approach, angiogenesis after implantation may be enhanced by incorporating endothelial cells, genetically modified cells, or specific factors (including vascular endothelial growth factor) into the scaffold or exposing the graft to a hypoxic environment just before implantation. Better understanding of the roles of hypoxia will help prevent common problems and exploit potential benefits of hypoxia in engineered tissues.</p>","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 9","pages":"2153-62"},"PeriodicalIF":0.0000,"publicationDate":"2007-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2006.0417","citationCount":"277","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/ten.2006.0417","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 277

Abstract

Oxygen is a potent modulator of cell function and wound repair in vivo. The lack of oxygen (hypoxia) can create a potentially lethal environment and limit cellular respiration and growth or, alternatively, enhance the production of the specific extracellular matrix components and increase angiogenesis through the hypoxia-inducible factor-1 pathway. For the in vitro generation of clinically relevant tissue-engineered grafts, these divergent actions of hypoxia should be addressed. Diffusion through culture medium and tissue typically limits oxygen transport in vitro, leading to hypoxic regions and limiting the viable tissue thickness. Approaches to overcoming the transport limitations include culture with bioreactors, scaffolds with artificial microvasculature, oxygen carriers, and hyperbaric oxygen chambers. As an alternate approach, angiogenesis after implantation may be enhanced by incorporating endothelial cells, genetically modified cells, or specific factors (including vascular endothelial growth factor) into the scaffold or exposing the graft to a hypoxic environment just before implantation. Better understanding of the roles of hypoxia will help prevent common problems and exploit potential benefits of hypoxia in engineered tissues.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
缺氧在体外组织工程中的作用。
氧是体内细胞功能和伤口修复的有效调节剂。缺氧可以创造一个潜在的致命环境,限制细胞呼吸和生长,或者,通过缺氧诱导因子-1途径,增强特定细胞外基质成分的产生和增加血管生成。为了在体外产生临床相关的组织工程移植物,应该解决这些缺氧的不同作用。通过培养基和组织的扩散通常限制体外氧运输,导致缺氧区域和限制活组织厚度。克服转运限制的方法包括生物反应器培养、人工微血管支架、氧气载体和高压氧舱。作为一种替代方法,植入后血管生成可以通过将内皮细胞、基因修饰细胞或特定因子(包括血管内皮生长因子)加入支架或在植入前将移植物暴露于缺氧环境中来增强。更好地了解缺氧的作用将有助于预防常见问题,并在工程组织中利用缺氧的潜在益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
自引率
0.00%
发文量
0
期刊最新文献
Cartilage reshaping via in vitro mechanical loading. ECM molecules mediate both Schwann cell proliferation and activation to enhance neurite outgrowth. Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics. Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduits. A novel time-varying poly lactic-co glycolic acid external sheath for vein grafts designed under physiological loading.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1