Development of a novel bioreactor for the mechanical loading of tissue-engineered heart muscle.

R K Birla, Y C Huang, R G Dennis
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引用次数: 69

Abstract

Objective: In this study, we describe a novel bioreactor system to deliver controlled stretch protocols to bioengineered heart muscle (BEHM) constructs. Our primary objective was to evaluate the effect of mechanical stretch on the contractile properties of three-dimensional cardiac constructs in vitro.

Methods: BEHMs were formed by culturing primary neonatal cardiac myocytes in a fibrin gel using a method previously developed in our laboratory. A custom bioreactor system was designed using SolidWorks (Concord, MA) and structural components were manufactured using fusion deposition modeling. We utilized the bioreactor to evaluate the effect of 2-, 6-, and 24-hour stretch protocols on the stretch-induced changes in contractile function of BEHMs.

Results: We were able to demonstrate compatibility of the bioreactor system with BEHMs and were able to stretch all the constructs with zero incidence of failure. We found that loading the constructs for 2, 6, and 24 hours during a 24-hour period using a stretch protocol of 1 Hz, 10% stretch did not result in any significant change in the active force, specific force, pacing characteristics, and morphological features.

Conclusions: In this study, we demonstrate compatibility of a novel bioreactor system with BEHMs and the stability of the BEHMs in response to stretch protocols.

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用于组织工程心肌机械负荷的新型生物反应器的开发。
目的:在这项研究中,我们描述了一种新的生物反应器系统,以提供生物工程心肌(BEHM)结构的受控拉伸协议。我们的主要目的是评估机械拉伸对体外三维心脏结构收缩特性的影响。方法:使用我们实验室先前开发的方法,在纤维蛋白凝胶中培养初生新生儿心肌细胞形成behm。使用SolidWorks (Concord, MA)设计了定制的生物反应器系统,并使用融合沉积建模制造了结构部件。我们利用生物反应器来评估2-、6-和24小时拉伸方案对拉伸引起的BEHMs收缩功能变化的影响。结果:我们能够证明生物反应器系统与BEHMs的兼容性,并且能够以零失败率拉伸所有结构。我们发现,在24小时的时间内,使用1 Hz的拉伸方案,将结构体加载2、6和24小时,10%的拉伸不会导致主动力、比力、起搏特征和形态特征的任何显著变化。结论:在这项研究中,我们证明了一种新型生物反应器系统与behm的兼容性,以及behm在拉伸协议下的稳定性。
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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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