Design and development of a flow injection-capillary electrophoresis analyzer employing fiber optic detection.

Grady Hanrahan, Florence Tse, Froseen T Dahdouh, Keith Clarke, Frank A Gomez
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Abstract

The development and experimental optimization of a novel flow injection-capillary electrophoresis (FI-CE) analyzer employing UV-visible fiber optic detection is described. The analyzer incorporates a miniature charge-coupled device (CCD) spectrometer and operates in a graphical programming environment. Data from experimental optimization studies and small molecule separations involving affinity capillary electrophoresis (ACE) and indirect detection of anions are presented. Future directions in terms of instrument automation and incorporation into a microfluidic format are also discussed.

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光纤检测流动注射-毛细管电泳分析仪的设计与研制。
介绍了一种新型紫外可见光纤流动注射-毛细管电泳(FI-CE)分析仪的研制和实验优化。该分析仪集成了一个微型电荷耦合器件(CCD)光谱仪,并在图形编程环境中操作。从实验优化研究和小分子分离涉及亲和毛细管电泳(ACE)和间接检测阴离子的数据。还讨论了仪器自动化和集成到微流控格式方面的未来方向。
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