Determination of active ingredients in corn silk, leaf, and kernel by capillary electrophoresis with electrochemicaI detection.

Miao Lin, Qing-Cui Chu, Xiu-Hui Tian, Jian-Nong Ye
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Abstract

Corn has been known for its accumulation of flavones and phenolic acids. However, many parts of corn, except kernel, have not drawn much attention. In this work, a method based on capillary zone electrophoresis with electrochemical detection has been used for the separation and determination of epicatechin, rutin, ascorbic acid (Vc), kaempferol, chlorogenic acid, and quercetin in corn silk, leaf, and kernel. The distribution comparison of the ingredients among silk, leaf, and kernel is discussed. Several important factors--including running buffer acidity, separation voltage, and working electrode potential--were evaluated to acquire the optimum analysis conditions. Under the optimum conditions, the analytes could be well separated within 19 min in a 40-mmol/L borate buffer (pH 9.2). The response was linear over three orders of magnitude with detection limits (S/N = 3) ranging from 4.97 x 10(-8) to 9.75 x 10(-8) g/mL. The method has been successfully applied for the analysis of corn silk, leaf, and kernel with satisfactory results.

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毛细管电泳-电化学检测法测定玉米蚕丝、叶片和籽粒中的有效成分。
玉米以其黄酮和酚酸的积累而闻名。然而,玉米的许多部分,除了籽粒,没有引起太多的关注。采用毛细管区带电泳-电化学检测的方法分离测定了玉米蚕丝、叶片和籽粒中表儿茶素、芦丁、抗坏血酸、山奈酚、绿原酸和槲皮素的含量。对蚕丝、蚕叶、蚕仁的成分分布进行了比较。评估了几个重要因素,包括运行缓冲液酸度、分离电压和工作电极电位,以获得最佳分析条件。在最佳条件下,40 mmol/L硼酸盐缓冲液(pH 9.2)在19 min内分离效果良好。检测限(S/N = 3)为4.97 × 10(-8) ~ 9.75 × 10(-8) g/mL。该方法已成功地应用于玉米丝、玉米叶和玉米仁的分析,结果令人满意。
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