Structural analysis of a fucoidan from the brown alga Fucus evanescens by MALDI-TOF and tandem ESI mass spectrometry

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Carbohydrate Research Pub Date : 2009-04-21 DOI:10.1016/j.carres.2009.01.023

Stanislav D. Anastyuk, Natalia M. Shevchenko, Evgeny L. Nazarenko, Pavel S. Dmitrenok, Tatyana N. Zvyagintseva

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引用次数: 83

Abstract

A fucoidan, a heterogeneous sulfated polysaccharide from the brown alga Fucus evanescens, was depolymerized under solvolytic conditions, and its ethanol-extracted low-molecular-weight fraction was analyzed by MALDI-TOFMS and ESIMS/MS. It was found that the mixture contained unsulfated oligosaccharides including some monosulfated components, which were shown to consist of mainly (1→3)-linked 2-O-sulfonated fucose residues (from 1 to 4). Minor components of the mixture were shown to contain 2-O- and 4-O-sulfonated xylose and galactose residues. Among them, mixed monosulfonated fucooligosaccharides were detected and characterized: Xyl-(1→4)-Fuc, Gal-(1→4)-Fuc, Gal-(1→4)-Gal-(1→4)-Fuc, Gal-(1→4)-Gal. Fucose, galactose, and xylose residues were shown to be mainly 2-O-sulfonated with traces of 4-O-sulfonation. Glucuronic acid was also found as a part of non-sulfated fucooligosaccharides: Fuc-(1→3)-GlcA, Fuc-(1→4)-Fuc-(1→3)-GlcA, Fuc-(1→3)-Fuc-(1→4)-Fuc-(1→3)-GlcA.

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MALDI-TOF和ESI串联质谱分析褐藻褐藻聚糖的结构

采用溶剂解聚法对褐藻褐藻多糖进行解聚，并利用MALDI-TOFMS和ESIMS/MS对其乙醇提取的低分子量组分进行分析。结果表明，该混合物含有非磺化低聚糖，其中包括一些单磺化成分，主要由(1→3)连接的2-O-磺化焦残基(从1到4)组成。混合物的次要成分含有2-O-和4- o -磺化木糖和半乳糖残基。其中，检测并表征了混合单磺化岩藻低聚糖:Xyl-(1→4)- fuc、Gal-(1→4)- fuc、Gal-(1→4)-Gal-(1→4)- fuc、Gal-(1→4)-Gal-(1→4)- Gal。焦糖、半乳糖和木糖残基主要为2- o -磺化，少量为4- o -磺化。葡萄糖醛酸也被发现为非硫酸化的岩藻低聚糖的一部分:Fuc-(1→3)- glca, Fuc-(1→4)-Fuc-(1→3)- glca, Fuc-(1→3)-Fuc-(1→4)-Fuc-(1→3)- glca。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".