{"title":"Investigation of molecular recognition in biological systems using cellular membrane affinity chromatography.","authors":"Irving W Wainer","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Cellular membrane affinity chromatography (CMAC) columns have been created through the immobilization of cellular membrane fragments on liquid chromatographic supports. A CMAC column containing the human organic cation transporter, CMAC(hOCT1) column, has been used to study the stereoselective binding of competitive inhibitors. The chromatographic data obtained using the CMAC(hOCT1) column was to develop a pharmacophore model that described the stereoselectivity. The results indicate that a dynamic chiral recognition model based upon conformational adjustments between the inhibitors and hOCT1 is responsible for the observed steroeselectivity.</p>","PeriodicalId":10052,"journal":{"name":"Chimica Oggi-chemistry Today","volume":"26 5 Suppl","pages":"19-22"},"PeriodicalIF":0.0000,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678806/pdf/nihms78847.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chimica Oggi-chemistry Today","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Chemistry","Score":null,"Total":0}
引用次数: 0
Abstract
Cellular membrane affinity chromatography (CMAC) columns have been created through the immobilization of cellular membrane fragments on liquid chromatographic supports. A CMAC column containing the human organic cation transporter, CMAC(hOCT1) column, has been used to study the stereoselective binding of competitive inhibitors. The chromatographic data obtained using the CMAC(hOCT1) column was to develop a pharmacophore model that described the stereoselectivity. The results indicate that a dynamic chiral recognition model based upon conformational adjustments between the inhibitors and hOCT1 is responsible for the observed steroeselectivity.
期刊介绍：
Chimica Oggi – Chemistry Today is a peer reviewed, bimonthly journal, of the TKS TeknoScienze Publisher.
It deals with Fine Chemicals, Applied Chemistry and Biotechnology.
Founded in 1983 Chimica Oggi – Chemistry Today soon became a leading journal in linking industry and academia and gained an immediate appreciation worldwide.
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