The role of CC chemokine receptor 2 on microglia activation and blood-borne cell recruitment after transient focal cerebral ischemia in mice

Abstract

The chemokine receptor 2 (CCR2) is involved in inflammatory reactions following cerebral ischemia. Monocyte chemoattractant protein-1 (MCP-1) binds with high affinity to CCR2. MCP-1 is necessary for recruiting blood-borne cells to the injury site whereas it does not affect microglia activation and migration. MCP-1-deficient mice develop smaller infarcts and show a better functional outcome. CCR2-deficient mice also develop smaller infarcts and have a reduced expression of inflammatory cytokines during reperfusion. In the present study we investigated the differential role of inflammatory cells in CCR2-deficient mice, using green fluorescent protein (GFP)-transgenic bone marrow chimeras. After 30 min of transient middle cerebral artery occlusion (MCAO), activation of local microglia was similar in CCR2-deficient animals and their littermate controls over the study period, whereas an influx of GFP-positive cells was diminished in CCR2-deficient mice. Infiltrating macrophages were significantly reduced at day seven in the deficient animals (26.04 ± 25.19 cells/mm²) compared to control mice (86.83 ± 44.41 cells/mm², p < 0.001). Neutrophils were also significantly reduced in CCR2-deficient mice (83% on day 2, 76% on day 4 and 89% on day 7, p < 0.001). A significant reduction of infarct volume in CCR2-deficient animals could not be detected. In this study a clear differentiation of local and blood-borne inflammatory cell reaction after cerebral ischemia could be shown, demonstrating that CCR2-deficiency attenuates hematogenous cell recruitment to the injury site whereas microglia activation and migration is not affected.