[Detection situation of an adhesion factor in diarrheagenic Escherichia coli].

Shinobu Murakami, Hitoshi Miyamoto, Mitsuharu Murase
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Abstract

Diarrhea caused by the Escherichia coli with held adhesion came to attention. We performed an adhesion-related gene and relation of diarrhea. Subjects were 77 outpatients with diarrhea from June 2003 to December 2005. A total of 102 E. coli strains randomly isolated from stool specimens. All the toxigenic examinations were negative, and there were not the relations. Adhesion-related gene were 10 strains found. As for the contents, astA was 5 strains, 2 strains of aggR, 2 strains of eaeA, 1 strain of eaeA plus astA. Of these, we were able to classify 5 strains in serological typing, but remain 5 strains did not typing. Only one strain of O157 was VT positive. There is not it with causative E. coli of diarrhea even if serological typing is negative. Therefore it was thought that an adhesion-related gene test was important.

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致泻性大肠杆菌中某粘附因子的检测情况
粘连性大肠杆菌引起的腹泻引起了人们的注意。我们检测了粘连相关基因与腹泻的关系。研究对象为2003年6月至2005年12月77例腹泻门诊患者。从粪便标本中随机分离出102株大肠杆菌。所有产毒检查均为阴性,两者之间无相关性。发现黏附相关基因10株。在含量方面,astA为5株,aggR为2株,eaeA为2株,eaeA + astA为1株。其中,我们能够对5株进行血清学分型,但仍有5株未分型。只有1株O157呈VT阳性。即使血清学分型为阴性,腹泻致病性大肠杆菌也不存在。因此,认为黏附相关基因检测是重要的。
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