Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus.

Veterinary Therapeutics Pub Date : 2009-01-01
Mohamed Abd-Eldaim, Melissa J Beall, Melissa A Kennedy
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Abstract

Feline panleukopenia virus (FPV) is a significant pathogen of cats. Rapid virus detection is critical for treatment and management, especially in populations in which spread may occur. This study investigated the ability of the SNAP Canine Parvovirus Antigen Test Kit (SNAP Parvo, IDEXX Laboratories) to detect FPV with confirmation of viral identity by polymerase chain reaction (PCR) assay and genetic sequencing on fecal samples (n = 97) from cats with suspected FPV infection. Fifty-five samples were positive by SNAP Parvo; 54 of 55 were also positive by conventional PCR assay and were identified as FPV by genetic sequencing. This study demonstrates that SNAP Parvo can detect FPV in clinical samples.

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商用犬细小病毒ELISA检测猫泛白细胞减少症病毒。
猫泛白细胞减少病毒(FPV)是猫的一种重要病原体。快速检测病毒对于治疗和管理至关重要,特别是在可能发生传播的人群中。本研究研究了SNAP犬细小病毒抗原检测试剂盒(SNAP Parvo, IDEXX实验室)检测FPV的能力,并通过聚合酶链反应(PCR)测定和基因测序对疑似感染FPV的猫粪便样本(n = 97)进行病毒鉴定。55份样品SNAP Parvo阳性;55例中有54例经常规PCR检测呈阳性,经基因测序鉴定为FPV。本研究证明SNAP Parvo可以检测临床样品中的FPV。
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