{"title":"Antidiarrheal evaluation of the ethanol extract of Nauclea latifolia root bark.","authors":"O J Owolabi, Z A M Nworgu, K Odushu","doi":"10.1358/mf.2010.32.8.1440747","DOIUrl":null,"url":null,"abstract":"<p><p>Nauclea latifolia (Rubiaceae) is a medicinal plant used in Nigeria folk medicine for the treatment of gastrointestinal disorders. The root bark of the plant was extracted with 70% ethanol and the extract screened for antidiarrheal activity by investigating castor oil-induced diarrhea and small intestinal motility in mice. The effects of the extract on isolated rat ileum were also investigated. The extract (125, 250 and 500 mg/kg) caused a significant decrease in the frequency of diarrhea and conferred protection following castor oil administration (P < 0.05). It also significantly (P < 0.0001) inhibited small intestinal motility in mice at the same doses. Its activity was dose-dependent and when compared to atropine, its antidiarrheal effects at 500 mg/kg were 179% and 165% respectively, in castor oil-induced diarrhea and small intestinal motility experiments. Extract effects on rat ileum revealed a significant (P < 0.0001) inhibition of acetylcholine-induced contractions at 0.2 and 2 mg/mL final bath concentrations used. These findings confirm the antidiarrheal activity of the root bark extract of N. latifolia.</p>","PeriodicalId":18443,"journal":{"name":"Methods and findings in experimental and clinical pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and findings in experimental and clinical pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1358/mf.2010.32.8.1440747","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Nauclea latifolia (Rubiaceae) is a medicinal plant used in Nigeria folk medicine for the treatment of gastrointestinal disorders. The root bark of the plant was extracted with 70% ethanol and the extract screened for antidiarrheal activity by investigating castor oil-induced diarrhea and small intestinal motility in mice. The effects of the extract on isolated rat ileum were also investigated. The extract (125, 250 and 500 mg/kg) caused a significant decrease in the frequency of diarrhea and conferred protection following castor oil administration (P < 0.05). It also significantly (P < 0.0001) inhibited small intestinal motility in mice at the same doses. Its activity was dose-dependent and when compared to atropine, its antidiarrheal effects at 500 mg/kg were 179% and 165% respectively, in castor oil-induced diarrhea and small intestinal motility experiments. Extract effects on rat ileum revealed a significant (P < 0.0001) inhibition of acetylcholine-induced contractions at 0.2 and 2 mg/mL final bath concentrations used. These findings confirm the antidiarrheal activity of the root bark extract of N. latifolia.