E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming.

IF 6.2 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Reports Pub Date : 2011-07-01 DOI:10.1038/embor.2011.88

Torben Redmer, Sebastian Diecke, Tamara Grigoryan, Angel Quiroga-Negreira, Walter Birchmeier, Daniel Besser

引用次数: 293

Abstract

We report new functions of the cell-adhesion molecule E-cadherin in murine pluripotent cells. E-cadherin is highly expressed in mouse embryonic stem cells, and interference with E-cadherin causes differentiation. During cellular reprogramming of mouse fibroblasts by OCT4, SOX2, KLF4 and c-MYC, fully reprogrammed cells were exclusively observed in the E-cadherin-positive cell population and could not be obtained in the absence of E-cadherin. Moreover, reprogrammed cells could be established by viral E-cadherin in the absence of exogenous OCT4. Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming.

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e -钙粘蛋白对胚胎干细胞的多能性至关重要，可以在体细胞重编程过程中取代OCT4。

我们报道了细胞粘附分子e -钙粘蛋白在小鼠多能细胞中的新功能。E-cadherin在小鼠胚胎干细胞中高度表达，干扰E-cadherin会导致分化。在OCT4、SOX2、KLF4和c-MYC对小鼠成纤维细胞进行细胞重编程时，只在E-cadherin阳性的细胞群中观察到完全重编程的细胞，在没有E-cadherin的情况下无法获得完全重编程的细胞。此外，在缺乏外源OCT4的情况下，病毒E-cadherin可以建立重编程细胞。因此，重编程需要与基本转录因子相互作用的空间线索。细胞粘附分子e -钙粘蛋白在多能性和重编程中具有重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

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