Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA

IF 1.9 2区农林科学 Q2 VETERINARY SCIENCES Journal of Feline Medicine and Surgery Pub Date : 2011-12-01 DOI:10.1016/j.jfms.2011.07.009

Brian A. DiGangi DVM, MS, DABVP , Lauren K. Gray BS , Julie K. Levy DVM, PhD, DACVIM , Edward J. Dubovi PhD , Sylvia J. Tucker BS

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引用次数: 27

Abstract

Serum antibody titers are a useful measurement of protection against infection (feline panleukopenia virus [FPV]) or clinical disease (feline herpesvirus-1 [FHV] and feline calicivirus [FCV]), and their determination has been recommended as part of disease outbreak management in animal shelters. The objective of this study was to determine the sensitivity, specificity, and inter-observer and inter-assay agreement of two semi-quantitative point-of-care assays for the detection of protective antibody titers (PAT) against FPV, FHV and FCV in shelter cats. Low sensitivity for FPV antibodies (28%) rendered a canine point-of-care assay inappropriate for use in cats. The feline point-of-care assay also had low sensitivity (49%) and low negative predictive value (74%) for FPV PAT detection, but was highly accurate in the assessment of FHV and FCV PAT. Improvements in accuracy and repeatability of FPV PAT determination could make this tool a valuable component of a disease outbreak response in animal shelters.

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使用即时护理ELISA检测收容所猫抗猫泛白细胞减少病毒、猫疱疹病毒-1和猫杯状病毒的保护性抗体滴度

血清抗体滴度是预防感染(猫泛白细胞减少病毒[FPV])或临床疾病(猫疱疹病毒-1 [FHV]和猫钙状病毒[FCV])的有效指标，其测定已被推荐作为动物收容所疾病暴发管理的一部分。本研究的目的是确定两种半定量护理点检测方法的敏感性、特异性以及观察者间和测定间的一致性，用于检测收容所猫对FPV、FHV和FCV的保护性抗体滴度(PAT)。对FPV抗体的低敏感性(28%)使得犬点护理检测不适合用于猫。猫护理点检测法对FPV - PAT检测的灵敏度也很低(49%)，阴性预测值也很低(74%)，但对FHV和FCV - PAT的评估非常准确。提高FPV PAT测定的准确性和可重复性，可使该工具成为动物收容所疾病暴发应对的重要组成部分。

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来源期刊

Journal of Feline Medicine and Surgery 农林科学-兽医学

CiteScore

3.90

自引率

17.60%

发文量

254

审稿时长

8-16 weeks

期刊介绍： JFMS is an international, peer-reviewed journal aimed at both practitioners and researchers with an interest in the clinical veterinary healthcare of domestic cats. The journal is published monthly in two formats: ‘Classic’ editions containing high-quality original papers on all aspects of feline medicine and surgery, including basic research relevant to clinical practice; and dedicated ‘Clinical Practice’ editions primarily containing opinionated review articles providing state-of-the-art information for feline clinicians, along with other relevant articles such as consensus guidelines.