The effect of boric acid on acethylcholine, bethanechol and potasssium-evoked responses on ileum of rat

S. Ince, R. Turkmen, H. Yavuz
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引用次数: 6

Abstract

1 The aim of this study was to clarify the effect of boric acid on contractions of rat isolated ileum.

2 Contractile responses expressed as Emax and pD2 for acetylcholine (10−3–10−8m, Ach), bethanechol (10−3–10−8m) and potassium (10–80 × 10−3m, KCl) were determined in the absence and presence of boric acid (10−3; 5 × 10−4; 10−4m).

3 The contractile response to Ach in the presence of verapamil (10−6 or 10−8m) or in calcium-free Tyrode’s solution was also determined in the absence and presence of boric acid.

4 Boric acid did not affect the contractile response to Ach, bethanechol or KCl. Single or cumulative treatment of boric acid did not affect ileum muscle contraction evoked by KCl. The atropine-resistant component of Ach-induced contraction and 4-diphenyl-acetoxy-N-methyl-piperidine methiodide-resistant component of bethanechol-induced contraction were not inhibited by boric acid (10−3m). The contractile response to Ach was reduced in calcium-free Tyrode’s solution, and the contractile response was not affected by (10−8m). The addition of boric acid (10−3m) in combination with verapamil (10−8m) did not significantly affect the contractile response to Ach.

5 In conclusion, boric acid does not affect contractions induced by Ach, bethanechol or potassium in rat isolated ileum.

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硼酸对大鼠回肠乙酰胆碱、乙酰胆碱和钾诱发反应的影响
本研究的目的是阐明硼酸对大鼠离体回肠收缩的影响。2在不存在和存在硼酸(10−3;5 × 10−4;10−4米)。在维拉帕米(10−6或10−8 m)或无钙Tyrode’s溶液中,也测定了在没有硼酸和硼酸存在的情况下,乙酰胆碱的收缩反应。硼酸对乙酰胆碱、乙二酚和氯化钾的收缩反应没有影响。硼酸单次或累积处理对氯化钾引起的回肠肌收缩无影响。硼酸(10 ~ 3 m)对乙酰胆碱诱导的收缩的抗阿托品成分和4-二苯基-乙酰氧基- n -甲基哌啶抗甲氧胺成分没有抑制作用。在无钙Tyrode’s溶液中,对Ach的收缩反应降低,且(10−8 m)对收缩反应没有影响。硼酸(10 ~ 3 m)与异搏米(10 ~ 8 m)联合添加对乙酰胆碱的收缩反应无显著影响。5由此可见,硼酸不影响乙酰胆碱、亚丁酚或钾诱导的大鼠离体回肠收缩。
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Issue Information Autonomic and Autacoid Pharmacology: Goodbye and thank you Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue Retraction: Dopamine receptor immunohistochemistry in the rat choroid plexus. Issue Information
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