{"title":"An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film.","authors":"Merve Özdemir, Fatma Arslan, Halit Arslan","doi":"10.3109/10731199.2011.646411","DOIUrl":null,"url":null,"abstract":"<p><p>In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 (-7) - 1.0 × 10 (-6)M (R(2) = 0.997) and 1.0 × 10 (-5) - 1.0 × 10 (-3) M (R(2) = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.646411","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial cells, blood substitutes, and immobilization biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10731199.2011.646411","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/4/3 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 (-7) - 1.0 × 10 (-6)M (R(2) = 0.997) and 1.0 × 10 (-5) - 1.0 × 10 (-3) M (R(2) = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
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