Development and evaluation of an enzyme-linked immunosorbent assay for dengue capsid.

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-03-29 DOI:10.2174/1874357901206010029

Suganya Selvarajah, Udayan Chatterji, Richard Kuhn, Richard Kinney, Subhash G Vasudevan, Philippe Gallay

{"title":"Development and evaluation of an enzyme-linked immunosorbent assay for dengue capsid.","authors":"Suganya Selvarajah, Udayan Chatterji, Richard Kuhn, Richard Kinney, Subhash G Vasudevan, Philippe Gallay","doi":"10.2174/1874357901206010029","DOIUrl":null,"url":null,"abstract":"<p><p>The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.</p>","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"29-37"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/f9/TOVJ-6-29.PMC3322434.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Virology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874357901206010029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/3/29 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

登革热衣壳酶联免疫吸附试验的建立与评价。

登革热在世界各地传播的惊人速度及其感染的严重程度使登革热成为全世界人类生命的主要威胁。不幸的是，迄今为止还没有针对登革热的有效疫苗或治疗方法。由于只有少数检测方法能够快速灵敏地检测登革热，我们开发了一种特异性抗原捕获酶联免疫吸附试验(ELISA)，用于检测丰富的结构登革热-2衣壳蛋白。我们发现ELISA可以快速灵敏地检测登革热-2在包括人类和蚊子细胞在内的各种细胞系中的复制。使用抗衣壳抗体，我们证明了衣壳ELISA与其他具有良好特征的登革热检测方法(如细胞内FACS染色(IFSA)和荧光焦点(FFA)检测方法)一样准确。衣壳酶联免疫吸附试验不仅是体外基础研究的有用工具，而且也可能是登革热患者感染的有价值的诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

The Open Virology Journal

自引率

0.00%

发文量