Human Papillomavirus (HPV) Genotyping: Automation and Application in Routine Laboratory Testing.

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-11-30 DOI:10.2174/1874357901206010144

M Torres, L Fraile, Jm Echevarria, B Hernandez Novoa, M Ortiz

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引用次数: 22

Abstract

A large number of assays designed for genotyping human papillomaviruses (HPV) have been developed in the last years. They perform within a wide range of analytical sensitivity and specificity values for the different viral types, and are used either for diagnosis, epidemiological studies, evaluation of vaccines and implementing and monitoring of vaccination programs. Methods for specific genotyping of HPV-16 and HPV-18 are also useful for the prevention of cervical cancer in screening programs. Some commercial tests are, in addition, fully or partially automated. Automation of HPV genotyping presents advantages such as the simplicity of the testing procedure for the operator, the ability to process a large number of samples in a short time, and the reduction of human errors from manual operations, allowing a better quality assurance and a reduction of cost. The present review collects information about the current HPV genotyping tests, with special attention to practical aspects influencing their use in clinical laboratories.

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人乳头瘤病毒(HPV)基因分型:自动化及其在常规实验室检测中的应用。

在过去的几年中，已经开发了大量用于人乳头瘤病毒(HPV)基因分型的检测方法。它们在不同病毒类型的分析敏感性和特异性值范围内发挥作用，并用于诊断、流行病学研究、疫苗评估以及实施和监测疫苗接种计划。HPV-16和HPV-18的特定基因分型方法在筛查项目中也有助于预防宫颈癌。另外，一些商业测试是完全或部分自动化的。HPV基因分型的自动化具有以下优点:操作人员的测试程序简单，能够在短时间内处理大量样本，减少人工操作的人为错误，从而更好地保证质量并降低成本。本综述收集了有关当前HPV基因分型检测的信息，特别关注影响其在临床实验室使用的实际方面。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Open Virology Journal

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