Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry.

Abstract

A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4-1.6 μg mL(-1) (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% (n = 4). The detection limit was obtained, 0.26 μg mL(-1) (1.7 μM). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.