Assessment of tracer 99mTc(V)-DMSA uptake as a measure of tumor cell proliferation in vitro.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2013-01-01 Epub Date: 2013-01-15 DOI:10.1371/journal.pone.0054361

Fatma J Al-Saeedi, Princy M Mathew, Yunus A Luqmani

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引用次数: 6

Abstract

Purpose: To examine whether (99m)Tc(V)-DMSA could be used as a non-invasive measure of cancer cell proliferation.

Methods: Human breast cancer MCF-7, MDA-MB-231 and pII, and prostate cancer PC-3 cell lines were grown to 30, 50 and 100% confluency and pulsed with (99m)Tc(V)-DMSA in media for 60 min at 37°C. DNA synthesis was analysed by quantification of the S phase using flow cytometry, [methyl-(3)H]thymidine incorporation and expression of proliferation markers PCNA and Ki-67 using realtime PCR. One way ANOVA was used to compare groups.

Results: In all cell lines rates of (99m)Tc(V)-DMSA uptake were inversely related to cell density. This was paralleled by similar trends in S phase proportions, [methyl-(3)H]thymidine incorporation and expression of PCNA and Ki-67.

Conclusion: Rates of (99m)Tc(V)-DMSA uptake into different types of tumour cells correlate well with cell density that is useful as a non-invasive measure of tumour cellular proliferation in vivo.

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示踪剂99mTc(V)-DMSA摄取作为肿瘤细胞体外增殖指标的评估。

目的:探讨(99m)Tc(V)-DMSA是否可以作为癌细胞增殖的无创指标。方法:将人乳腺癌MCF-7、MDA-MB-231、pII和前列腺癌PC-3细胞系培养至30%、50%和100%的融合度，在37℃的培养基中用(99m)Tc(V)-DMSA脉冲培养60 min。流式细胞术定量分析S相DNA合成，[甲基-(3)H]胸腺嘧啶掺入，real - time PCR检测增殖标志物PCNA和Ki-67的表达。采用单因素方差分析进行组间比较。结果:在所有细胞系中，(99m)Tc(V)-DMSA摄取率与细胞密度呈负相关。S相比例、[甲基-(3)H]胸苷的掺入以及PCNA和Ki-67的表达也有类似的趋势。结论:(99m)Tc(V)-DMSA进入不同类型肿瘤细胞的速率与细胞密度密切相关，细胞密度是体内肿瘤细胞增殖的一种无创测量方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.