Fatma J Al-Saeedi, Princy M Mathew, Yunus A Luqmani
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引用次数: 6
Abstract
Purpose: To examine whether (99m)Tc(V)-DMSA could be used as a non-invasive measure of cancer cell proliferation.
Methods: Human breast cancer MCF-7, MDA-MB-231 and pII, and prostate cancer PC-3 cell lines were grown to 30, 50 and 100% confluency and pulsed with (99m)Tc(V)-DMSA in media for 60 min at 37°C. DNA synthesis was analysed by quantification of the S phase using flow cytometry, [methyl-(3)H]thymidine incorporation and expression of proliferation markers PCNA and Ki-67 using realtime PCR. One way ANOVA was used to compare groups.
Results: In all cell lines rates of (99m)Tc(V)-DMSA uptake were inversely related to cell density. This was paralleled by similar trends in S phase proportions, [methyl-(3)H]thymidine incorporation and expression of PCNA and Ki-67.
Conclusion: Rates of (99m)Tc(V)-DMSA uptake into different types of tumour cells correlate well with cell density that is useful as a non-invasive measure of tumour cellular proliferation in vivo.
目的:探讨(99m)Tc(V)-DMSA是否可以作为癌细胞增殖的无创指标。方法:将人乳腺癌MCF-7、MDA-MB-231、pII和前列腺癌PC-3细胞系培养至30%、50%和100%的融合度，在37℃的培养基中用(99m)Tc(V)-DMSA脉冲培养60 min。流式细胞术定量分析S相DNA合成，[甲基-(3)H]胸腺嘧啶掺入，real - time PCR检测增殖标志物PCNA和Ki-67的表达。采用单因素方差分析进行组间比较。结果:在所有细胞系中，(99m)Tc(V)-DMSA摄取率与细胞密度呈负相关。S相比例、[甲基-(3)H]胸苷的掺入以及PCNA和Ki-67的表达也有类似的趋势。结论:(99m)Tc(V)-DMSA进入不同类型肿瘤细胞的速率与细胞密度密切相关，细胞密度是体内肿瘤细胞增殖的一种无创测量方法。
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