Statins Protect the Blood Brain Barrier Acutely after Experimental Intracerebral Hemorrhage.

Journal of Behavioral and Brain Science Pub Date : 2013-02-01 Epub Date: 2012-12-10 DOI:10.4236/jbbs.2013.31010
Dongmei Yang, Robert A Knight, Yuxia Han, Kishor Karki, Jianfeng Zhang, Michael Chopp, Donald M Seyfried
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引用次数: 43

Abstract

Objectives: The goal of this study was to measure the impact of simvastatin and atorvastatin treatment on blood brain barrier (BBB) integrity after experimental intracerebral hemorrhage (ICH).

Methods: Primary ICH was induced in 27 male Wistar rats by stereotactic injection of 100 µL of autologous blood into the striatum. Rats were divided into three groups (n= 9/group): 1) oral treatment (2 mg/kg) of atorvastatin, 2) oral treatment (2 mg/kg) simvastatin, or 3) phosphate buffered saline daily starting 24-hours post-ICH and continuing daily for the next 3 days. On the fourth day, the animals underwent magnetic resonance imaging (MRI) for measurements of T1sat (a marker for BBB integrity), T2 (edema), and cerebral blood flow (CBF). After MRI, the animals were sacrificed and immunohistology or Western blotting was performed.

Results: MRI data for animals receiving simvastatin treatment showed significantly reduced BBB dysfunction and improved CBF in the ICH rim compared to controls (P<0.05) 4 days after ICH. Simvastatin also significantly reduced edema (T2) in the rim at 4 days after ICH (P<0.05). Both statin-treated groups demonstrated increased occludin and endothelial barrier antigen levels within the vessel walls, indicating better preservation of BBB function (P<0.05) and increased number of blood vessels (P<0.05).

Conclusions: Simvastatin treatment administered acutely after ICH protects BBB integrity as measured by MRI and correlative immunohistochemistry. There was also evidence of improved CBF and reduced edema by MRI. Conversely, atorvastatin showed a non-significant trend by MRI measurement.

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他汀类药物对实验性脑出血后血脑屏障的保护作用。
目的:本研究的目的是测量辛伐他汀和阿托伐他汀治疗对实验性脑出血(ICH)后血脑屏障(BBB)完整性的影响。方法:27只雄性Wistar大鼠,纹状体内立体定向注射100µL自体血,诱导原发性脑出血。将大鼠分为3组(n= 9/组):1)口服阿托伐他汀(2mg /kg), 2)口服辛伐他汀(2mg /kg),或3)从脑出血后24小时开始,连续3天每天磷酸缓冲盐水。第四天,动物接受磁共振成像(MRI)测量T1sat(血脑屏障完整性的标志)、T2(水肿)和脑血流量(CBF)。MRI后处死动物,进行免疫组织或免疫印迹检测。结果:在脑出血后4天,接受辛伐他汀治疗的动物的MRI数据显示,与对照组(P2)相比,脑出血边缘的血脑屏障功能障碍明显减少,脑血流功能改善(ppp)。结论:通过MRI和相关免疫组织化学测量,脑出血后急性给予辛伐他汀治疗可保护血脑屏障完整性。MRI也有改善脑血流和减少水肿的证据。相反,通过MRI测量,阿托伐他汀显示无显著趋势。
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