Tramadol inhibits the contractility of isolated human myometrium

N. H. Shah, E. Thomas, R. Jose, J. Peedicayil
{"title":"Tramadol inhibits the contractility of isolated human myometrium","authors":"N. H. Shah,&nbsp;E. Thomas,&nbsp;R. Jose,&nbsp;J. Peedicayil","doi":"10.1111/aap.12003","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n \n </p><ol>\n \n <li>This study was conducted to determine whether the atypical opioid analgesic tramadol inhibits the contractility of isolated non-pregnant human myometrium. Ten strips of non-pregnant human myometrium stimulated with 55 m<span>m</span> potassium chloride (KCl) were treated with three concentrations (30, 100 and 300 μ<span>m</span>) of tramadol to test for any inhibitory effect of tramadol. The effects of concurrent administration of the ß adrenoceptor antagonist propranolol (1 μ<span>m</span>), the guanylyl cyclase and nitric oxide synthase inhibitor methylene blue (20 μ<span>m</span>) and the opioid receptor antagonist naloxone (100 μ<span>m</span>) with tramadol were also studied.</li>\n \n <li>Tramadol caused a concentration-dependent inhibition of KCl-induced myometrial contractility, which was statistically significant at all three concentrations of tramadol used. Propranolol significantly reversed the inhibitory effect of 100 μ<span>m</span> tramadol on KCl-induced myometrial contractility but not that of 300 μ<span>m</span> tramadol. Neither methylene blue nor naloxone reversed the inhibitory effect of tramadol on KCl-induced myometrial contractility.</li>\n \n <li>These results suggest that tramadol inhibits KCl-induced contractility of isolated human myometrium. They also suggest that tramadol relaxes the myometrium due to stimulation of ß<sub>1</sub> adrenoceptors. However, the concentrations of tramadol required to relax the myometrium were high and likely to be attained at toxic doses, rather than therapeutic doses, of tramadol.</li>\n </ol>\n \n </div>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/aap.12003","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Autonomic and Autacoid Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aap.12003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15

Abstract

  1. This study was conducted to determine whether the atypical opioid analgesic tramadol inhibits the contractility of isolated non-pregnant human myometrium. Ten strips of non-pregnant human myometrium stimulated with 55 mm potassium chloride (KCl) were treated with three concentrations (30, 100 and 300 μm) of tramadol to test for any inhibitory effect of tramadol. The effects of concurrent administration of the ß adrenoceptor antagonist propranolol (1 μm), the guanylyl cyclase and nitric oxide synthase inhibitor methylene blue (20 μm) and the opioid receptor antagonist naloxone (100 μm) with tramadol were also studied.
  2. Tramadol caused a concentration-dependent inhibition of KCl-induced myometrial contractility, which was statistically significant at all three concentrations of tramadol used. Propranolol significantly reversed the inhibitory effect of 100 μm tramadol on KCl-induced myometrial contractility but not that of 300 μm tramadol. Neither methylene blue nor naloxone reversed the inhibitory effect of tramadol on KCl-induced myometrial contractility.
  3. These results suggest that tramadol inhibits KCl-induced contractility of isolated human myometrium. They also suggest that tramadol relaxes the myometrium due to stimulation of ß1 adrenoceptors. However, the concentrations of tramadol required to relax the myometrium were high and likely to be attained at toxic doses, rather than therapeutic doses, of tramadol.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
曲马多抑制离体人肌层的收缩性
本研究旨在确定非典型阿片类镇痛药曲马多是否能抑制离体非妊娠人肌层的收缩性。采用55 mm氯化钾(KCl)刺激10条未怀孕的人子宫肌层,用3种浓度(30、100和300 μm)的曲马多处理,观察曲马多的抑制作用。同时研究了β肾上腺素受体拮抗剂普萘洛尔(1 μm)、冠酰环化酶和一氧化氮合酶抑制剂亚甲基蓝(20 μm)和阿片受体拮抗剂纳洛酮(100 μm)与曲马多同时给药的效果。曲马多引起的kcl诱导的子宫肌力的浓度依赖性抑制,这在使用的所有三种浓度的曲马多具有统计学意义。普萘洛尔能显著逆转100 μm曲马多对kcl诱导的子宫肌力的抑制作用,而300 μm曲马多则不能。亚甲蓝和纳洛酮均不能逆转曲马多对氯化钾诱导的子宫肌收缩性的抑制作用。这些结果表明曲马多抑制kcl诱导的离体人肌层收缩性。他们还认为曲马多通过刺激ß1肾上腺素受体而使肌层松弛。然而,松弛肌层所需的曲马多浓度很高,而且可能在曲马多的中毒剂量而不是治疗剂量下达到。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Issue Information Autonomic and Autacoid Pharmacology: Goodbye and thank you Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue Retraction: Dopamine receptor immunohistochemistry in the rat choroid plexus. Issue Information
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1