Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro.

The Open Virology Journal Pub Date : 2013-04-05 Print Date: 2013-01-01 DOI:10.2174/1874357901307010049

M Hodik, A Lukinius, O Korsgren, G Frisk

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引用次数: 21

Abstract

Human Enteroviruses (HEVs) have been implicated in human pancreatic diseases such as pancreatitis and type 1 diabetes (T1D). Human studies are sparse or inconclusive and our aim was to investigate the tropism of two strains of Coxsackie B virus 5 (CBV-5) in vitro to primary human pancreatic cells. Virus replication was measured with TCID50 titrations of aliquots of the culture medium at different time points post inoculation. The presence of virus particles or virus proteins within the pancreatic cells was studied with immunohistochemistry (IHC) and electron microscopy (EM). None of the strains replicated in the human exocrine cell clusters, in contrast, both strains replicated in the endocrine islets of Langerhans. Virus particles were found exclusively in the endocrine cells, often in close association with insulin granules. In conclusion, CBV-5 can replicate in human endocrine cells but not in human exocrine cells, thus they might not be the cause of pancreatitis in humans. The association of virus with insulin granules might reflect the use of these as replication scaffolds.

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两株柯萨奇B5病毒株的趋向性分析显示病毒在人原代胰岛中生长，但在体外外分泌细胞簇中不生长。

人类肠病毒(hev)与胰腺炎和1型糖尿病(T1D)等人类胰腺疾病有关。人类研究很少或不确定，我们的目的是研究两株柯萨奇B病毒5 (CBV-5)在体外对原代人胰腺细胞的趋向性。在接种后不同时间点，用TCID50滴定培养基的等份来测定病毒的复制。用免疫组织化学(IHC)和电子显微镜(EM)研究了胰腺细胞内病毒颗粒或病毒蛋白的存在。没有一种菌株在人类外分泌细胞群中复制，相反，两种菌株在朗格汉斯的内分泌胰岛中复制。病毒颗粒仅在内分泌细胞中发现，通常与胰岛素颗粒密切相关。综上所述，CBV-5可以在人类内分泌细胞中复制，但不能在人类外分泌细胞中复制，因此它们可能不是人类胰腺炎的原因。病毒与胰岛素颗粒的关联可能反映了它们作为复制支架的使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Open Virology Journal

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