Mitochondrial DNA polymorphism in genes encoding ND1, COI and CYTB in canine malignant cancers.

Mitochondrial Dna Pub Date : 2015-06-01 Epub Date: 2013-10-09 DOI:10.3109/19401736.2013.840594

Brygida Slaska, Ludmila Grzybowska-Szatkowska, Sylwia Nisztuk, Magdalena Surdyka, Dorota Rozanska

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引用次数: 17

Abstract

The aim of the study was to identify DNA changes in mitochondrial gene fragments: NADH dehydrogenase subunit 1 (ND1), cytochrome c oxidase subunit I (COI) and cytochrome b (CYTB) in tumor tissue, normal tissue and blood, and to define their association with the tumor type in dogs. Molecular analysis included 144 tests in total. A functional effect of the non-synonymous protein coding SNP was predicted. The presence of polymorphisms in all tested gene fragments in individual tissues of dogs was observed. Heteroplasmic changes were found in ND1 and CYTB in epithelioma glandulae sebacei and in CYTB in lymphoma centroblasticum. The results of in silico analysis show the impact of these alleles (COI: 507, ND1: 450, 216, CYTB: 748) on the functioning of proteins and thus their potential role in carcinogenesis. The possible harmful effects of changes in polypeptides in positions T193N, V98M, V118M and H196P were evaluated. It seems that polymorphisms occurring in cells can have a negative impact on functioning of proteins. This promotes disorders of the energy level in cells.

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犬恶性肿瘤ND1、COI和CYTB基因的线粒体DNA多态性研究

本研究旨在鉴定犬肿瘤组织、正常组织和血液中线粒体基因片段NADH脱氢酶亚基1 (ND1)、细胞色素c氧化酶亚基I (COI)和细胞色素b (CYTB)的DNA变化，并确定其与肿瘤类型的关系。分子分析共144项。预测非同义蛋白编码SNP的功能效应。在狗的个体组织中观察到所有被测基因片段存在多态性。皮脂腺上皮瘤的ND1和CYTB呈异质改变，淋巴瘤中原心细胞的CYTB呈异质改变。硅分析结果显示这些等位基因(COI: 507, ND1: 450, 216, CYTB: 748)对蛋白质功能的影响，从而表明它们在致癌作用中的潜在作用。对T193N、V98M、V118M和H196P位置多肽变化可能产生的有害影响进行了评价。似乎细胞中发生的多态性会对蛋白质的功能产生负面影响。这促进了细胞能量水平的紊乱。

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Mitochondrial Dna 生物-遗传学

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2.4 months

期刊介绍： Previously published under the title DNA Sequence (Vols 1-19.3), Mitochondrial DNA accepts original high-quality reports based on mapping, sequencing and analysis of mitochondrial DNA and RNA. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, medical genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The editorial board will also consider manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences.