Identification and characterization of ϕH111-1: A novel myovirus with broad activity against clinical isolates of Burkholderia cenocepacia.

Bacteriophage Pub Date : 2013-10-01 DOI:10.4161/bact.26649
Karlene H Lynch, Yongjie Liang, Leo Eberl, David S Wishart, Jonathan J Dennis
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引用次数: 5

Abstract

Characterization of prophages in sequenced bacterial genomes is important for virulence assessment, evolutionary analysis, and phage application development. The objective of this study was to identify complete, inducible prophages in the cystic fibrosis (CF) clinical isolate Burkholderia cenocepacia H111. Using the prophage-finding program PHAge Search Tool (PHAST), we identified three putative intact prophages in the H111 sequence. Virions were readily isolated from H111 culture supernatants following extended incubation. Using shotgun cloning and sequencing, one of these virions (designated ϕH111-1 [vB_BceM_ϕH111-1]) was identified as the infective particle of a PHAST-detected intact prophage. ϕH111-1 has an extremely broad host range with respect to B. cenocepacia strains and is predicted to use lipopolysaccharide (LPS) as a receptor. Bioinformatics analysis indicates that the prophage is 42,972 base pairs in length, encodes 54 proteins, and shows relatedness to the virion morphogenesis modules of AcaML1 and "Vhmllikevirus" myoviruses. As ϕH111-1 is active against a broad panel of clinical strains and encodes no putative virulence factors, it may be therapeutically effective for Burkholderia infections.

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一种新型肌病毒的鉴定和特性:对临床分离的结核杆菌具有广泛活性。
在测序的细菌基因组中表征噬菌体对毒力评估、进化分析和噬菌体应用开发具有重要意义。本研究的目的是在囊性纤维化(CF)临床分离物结核杆菌H111中鉴定完整的、可诱导的噬菌体。利用噬菌体寻找程序噬菌体搜索工具(PHAge Search Tool, PHAST),我们在H111序列中鉴定出三个假定完整的噬菌体。延长孵育后,病毒体很容易从h1n1培养上清中分离出来。利用霰弹枪克隆和测序技术,鉴定出其中一个病毒粒子(指定为ϕH111-1 [vB_BceM_ϕH111-1])是phast检测到的完整噬菌体的感染颗粒。相对于cenocepacia B.菌株而言, h111 -1具有极其广泛的宿主范围,并且预计使用脂多糖(LPS)作为受体。生物信息学分析表明,该噬菌体长度为42,972个碱基对,编码54个蛋白,与AcaML1和“vhmllike病毒”肌病毒的病毒粒子形态发生模块相关。由于ϕH111-1对广泛的临床菌株具有活性,并且不编码推定的毒力因子,因此它可能对伯克霍尔德菌感染具有治疗效果。
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