Testing a proposed paradigm shift in analysis of phage DNA packaging

P. Serwer, E. Wright
{"title":"Testing a proposed paradigm shift in analysis of phage DNA packaging","authors":"P. Serwer, E. Wright","doi":"10.1080/21597081.2016.1268664","DOIUrl":null,"url":null,"abstract":"ABSTRACT We argue that a paradigm shift is needed in the analysis of phage DNA packaging. We then test a prediction of the following paradigm shift-engendering hypothesis. The motor of phage DNA packaging has two cycles: (1) the well-known packaging ATPase-driven (type 1) cycle and (2) a proposed back-up, shell expansion/contraction-driven (type 2) cycle that reverses type 1 cycle stalls by expelling accidentally packaged non-DNA molecules. We test the prediction that increasing the cellular concentration of all macromolecules will cause packaging-active capsids to divert to states of hyper-expansion and contraction. We use a directed evolution-derived, 3-site phage T3 mutant, adapted to propagation in concentrated bacterial cytoplasm. We find this prediction correct while discovering novel T3 capsids previously obscure.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bacteriophage","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21597081.2016.1268664","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8

Abstract

ABSTRACT We argue that a paradigm shift is needed in the analysis of phage DNA packaging. We then test a prediction of the following paradigm shift-engendering hypothesis. The motor of phage DNA packaging has two cycles: (1) the well-known packaging ATPase-driven (type 1) cycle and (2) a proposed back-up, shell expansion/contraction-driven (type 2) cycle that reverses type 1 cycle stalls by expelling accidentally packaged non-DNA molecules. We test the prediction that increasing the cellular concentration of all macromolecules will cause packaging-active capsids to divert to states of hyper-expansion and contraction. We use a directed evolution-derived, 3-site phage T3 mutant, adapted to propagation in concentrated bacterial cytoplasm. We find this prediction correct while discovering novel T3 capsids previously obscure.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
测试在噬菌体DNA包装分析中提出的范式转变
我们认为在噬菌体DNA包装的分析中需要一个范式转变。然后,我们测试了以下范式转换产生假设的预测。噬菌体DNA包装的马达有两个周期:(1)众所周知的包装atp酶驱动(第1型)周期和(2)提议的备用壳膨胀/收缩驱动(第2型)周期,通过排出意外包装的非DNA分子来逆转第1型周期的停滞。我们测试了增加所有大分子的细胞浓度将导致包装活性衣壳转向超膨胀和超收缩状态的预测。我们使用定向进化衍生的3位点噬菌体T3突变体,适应于在浓缩的细菌细胞质中繁殖。我们发现这一预测是正确的,同时发现了以前不为人知的新型T3衣壳。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Phagebiotics in treatment and prophylaxis of healthcare-associated infections. Testing a proposed paradigm shift in analysis of phage DNA packaging Fecal microbiota transplantation to fight Clostridium difficile infections and other intestinal diseases Félix Hubert d'Herelle (1873–1949): History of a scientific mind My scientific life
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1