Genetic Diversity Based on Coat Protein of Papaya ringspot virus (Pathotype P) Isolates from Bangladesh.

Abstract

The coat protein (CP) sequences of twelve Papaya ringspot virus (PRSV) (pathotype-P) isolates from six major papaya growing areas were determined and compared with those of published PRSV. The CP coding region varied in size from 846-852 nucleotides, encoding a protein of 282-284 amino acids. Comparative CP sequence analysis revealed that the PRSV-P isolates originating from Bangladesh were divergent up to 14 % at amino acids level. Further, the isolates from Bangladesh shared 86-95 % amino acid sequence identity with those reported from rest 21 of the Asia and 83-93 % amino acid sequence identity with isolates from the other parts of the world. A number of KE repeats were observed in the N terminus of the CP coding region of all Bangladesh isolates. Phylogenetic branching pattern revealed that the PRSV-P isolates originating from Bangladesh formed a distinct clade from those from the rest of the world. This forms the first report on the genetic diversity of PRSV-P isolates from Bangladesh.