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{"title":"DNA Immunization","authors":"Shixia Wang, Shan Lu","doi":"10.1002/9780471729259.mc1803s31","DOIUrl":null,"url":null,"abstract":"<p>DNA immunization was discovered in early 1990s, and its use has been expanded from vaccine studies to a broader range of biomedical research areas, such as the generation of high-quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation, and gene gun. In addition, several common considerations related to DNA immunization are discussed. <i>Curr. Protoc. Microbiol</i>. 31:18.3.1-18.3.24. ©2013 by John Wiley & Sons, Inc.</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780471729259.mc1803s31","citationCount":"28","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780471729259.mc1803s31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
DNA immunization was discovered in early 1990s, and its use has been expanded from vaccine studies to a broader range of biomedical research areas, such as the generation of high-quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation, and gene gun. In addition, several common considerations related to DNA immunization are discussed. Curr. Protoc. Microbiol . 31:18.3.1-18.3.24. ©2013 by John Wiley & Sons, Inc.
DNA免疫
DNA免疫于20世纪90年代初被发现，其用途已从疫苗研究扩展到更广泛的生物医学研究领域，例如作为研究试剂生成高质量的多克隆和单克隆抗体。本单元介绍了三种常见的DNA免疫方法:针注射、电穿孔和基因枪。此外，还讨论了与DNA免疫有关的几个常见问题。咕咕叫。Protoc。Microbiol 31:18.3.1-18.3.24。©2013 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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