Differentiation of Hepatocytes from Pluripotent Stem Cells

Q2 Biochemistry, Genetics and Molecular Biology Current Protocols in Stem Cell Biology Pub Date : 2018-02-16 DOI:10.1002/9780470151808.sc01g04s26

Sunil K. Mallanna, Stephen A. Duncan

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引用次数: 96

Abstract

Differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells into hepatocyte-like cells provides a platform to study the molecular basis of human hepatocyte differentiation, to develop cell culture models of liver disease, and to potentially provide hepatocytes for treatment of end-stage liver disease. Additionally, hepatocyte-like cells generated from human pluripotent stem cells could serve as platforms for drug discovery, determination of pharmaceutical-induced hepatotoxicity, and evaluation of idiosyncratic drug-drug interactions. Here, we describe a step-wise protocol previously developed in our laboratory that facilitates the highly efficient and reproducible differentiation of human pluripotent stem cells into hepatocyte-like cells. Our protocol uses defined culture conditions and closely recapitulates key developmental events that are found to occur during hepatogenesis. Curr. Protoc. Stem Cell Biol. 26:1G.4.1-1G.4.13. © 2013 by John Wiley & Sons, Inc.

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多能干细胞与肝细胞的分化

人胚胎干细胞(ES)和诱导多能干细胞(iPS)分化为肝细胞样细胞，为研究人肝细胞分化的分子基础、建立肝脏疾病的细胞培养模型，以及为治疗终末期肝脏疾病提供肝细胞提供潜在的平台。此外，由人类多能干细胞产生的肝细胞样细胞可以作为药物发现、药物诱导肝毒性测定和特殊药物-药物相互作用评估的平台。在这里，我们描述了先前在我们实验室开发的一种分步方案，该方案促进了人类多能干细胞向肝细胞样细胞的高效和可重复分化。我们的方案使用确定的培养条件，并密切概括在肝发生过程中发现的关键发育事件。咕咕叫。Protoc。干细胞生物学。26:1 .4.1-1 .4.13。©2013 by John Wiley &儿子,Inc。

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Current Protocols in Stem Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Published in affiliation with the International Society for Stem Cell Research (ISSCR), Current Protocols in Stem Cell Biology (CPSC) covers the most fundamental protocols and methods in the rapidly growing field of stem cell biology. Updated monthly, CPSC will constantly evolve with thelatest developments and breakthroughs in the field. Drawing on the expertise of leading researchers from around the world, Current Protocols in Stem Cell Biology includes methods and insights that will enhance the progress of global research.