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{"title":"RNA Structure Analysis of Viruses Using SHAPE","authors":"Cecily P. Burrill, Raul Andino","doi":"10.1002/9780471729259.mc15h03s30","DOIUrl":null,"url":null,"abstract":"<p>Selective 2′ hydroxyl acylation analyzed by primer extension (SHAPE) provides a means to investigate RNA structure with better resolution and higher throughput than has been possible with traditional methods. We present several protocols, which are based on a variety of previously published methods and were adapted and optimized for the analysis of poliovirus RNA in the Andino laboratory. These include methods for nondenaturing RNA extraction, RNA modification and primer extension, and data processing in ShapeFinder. <i>Curr. Protoc. Microbiol</i>. 30:15H.3.1-15H.3.12. © 2013 by John Wiley & Sons, Inc.</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780471729259.mc15h03s30","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780471729259.mc15h03s30","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Selective 2′ hydroxyl acylation analyzed by primer extension (SHAPE) provides a means to investigate RNA structure with better resolution and higher throughput than has been possible with traditional methods. We present several protocols, which are based on a variety of previously published methods and were adapted and optimized for the analysis of poliovirus RNA in the Andino laboratory. These include methods for nondenaturing RNA extraction, RNA modification and primer extension, and data processing in ShapeFinder. Curr. Protoc. Microbiol . 30:15H.3.1-15H.3.12. © 2013 by John Wiley & Sons, Inc.
利用SHAPE分析病毒RNA结构
通过引物延伸(SHAPE)分析选择性2’羟基酰化为研究RNA结构提供了一种比传统方法具有更高分辨率和更高通量的手段。我们提出了几种方案，这些方案基于各种先前发表的方法，并针对Andino实验室的脊髓灰质炎病毒RNA分析进行了调整和优化。这些方法包括非变性RNA提取、RNA修饰和引物扩展以及ShapeFinder中的数据处理。咕咕叫。Protoc。Microbiol 30:15H.3.1-15H.3.12。©2013 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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