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{"title":"Purification of Fibronectin","authors":"Steven K. Akiyama","doi":"10.1002/0471143030.cb1005s60","DOIUrl":null,"url":null,"abstract":"<p>This unit describes the purification of the multifunctional adhesive glycoprotein fibronectin from plasma or of cell-derived fibronectin from cell surfaces and from conditioned medium. Fibronectin can be used in cell adhesion and migration assays, and can be obtained in relatively high purity using simple affinity chromatography techniques. <i>Curr. Protoc. Cell Biol</i>. 60:10.5.1-10.5.13. © 2013 by John Wiley & Sons, Inc.</p>","PeriodicalId":40051,"journal":{"name":"Current Protocols in Cell Biology","volume":"60 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/0471143030.cb1005s60","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/0471143030.cb1005s60","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
This unit describes the purification of the multifunctional adhesive glycoprotein fibronectin from plasma or of cell-derived fibronectin from cell surfaces and from conditioned medium. Fibronectin can be used in cell adhesion and migration assays, and can be obtained in relatively high purity using simple affinity chromatography techniques. Curr. Protoc. Cell Biol . 60:10.5.1-10.5.13. © 2013 by John Wiley & Sons, Inc.
纤维连接蛋白的纯化
本单元描述了从血浆中纯化多功能粘附糖蛋白纤维连接蛋白或从细胞表面和条件培养基中纯化细胞源性纤维连接蛋白。纤维连接蛋白可用于细胞粘附和迁移试验，并且可以通过简单的亲和层析技术获得相对高纯度的纤维连接蛋白。咕咕叫。Protoc。细胞生物学，60:10.5.1-10.5.13。©2013 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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