[Comparison of usefulness of commercial ELISA Virion/Serion, homemade ELISA and tube agglutination test in serodiagnosis of tularemia].

Medycyna doswiadczalna i mikrobiologia Pub Date : 2013-01-01

Waldemar Rastawicki, Natalia Wolaniuk

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Abstract

Introduction: Tularemia is a highly infectious zoonotic disease caused by Gram-negative bacterium Francisella tularensis. The microbiological diagnosis of tularemia is based on bacteriological, molecular and serological investigations. In the present study we compared of usefulness of commercial ELISA Virion/Serion, home-made ELISA and tube agglutination test in serodiagnosis of tularemia.

Methods: Serum samples from 57 patients with clinical symptoms of tularemia, 13 patients with yersiniosis and 20 blood donors were tested. The cut-off limit of IgA, IgG and IgM serum antibodies in home-made ELISA was set at mean antibody titer determined in sera of healthy blood donors exceeded by the three standard deviations. The cut-off for positivity in tube agglutination test was titers 25. The IgG and IgM antibodies to lipopolysaccharides of F. tularensis in Virion/Serion ELISA were measured and results interpreted according to the instructions by the manufacturer.

Results: The results of the study showed that 39 (68.4%) serum samples obtained from the patients suspected for tularemia were positive by tube agglutination test and Virion/Serion ELISA assay for IgG and IgM antibodies. Home-made ELISA was slightly more sensitive and detected the IgA/IgG antibodies in 42 (73.7%) and IgM antibodies in 39 (68.4%) of serum samples. The positive reactions were not detected by the tube agglutination test and home-made ELISA in serum samples from patients with yersiniosis and blood donors. The Virion/Serion ELISA detected IgG antibodies in diagnostically significant level only in one blood donor.

Conclusions: In conclusion, all three serological tests can be successfully used in routine serodiagnosis of tularemia.

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市售酶联免疫吸附试验(ELISA)、自制酶联免疫吸附试验(ELISA)和试管凝集试验在兔吸虫病血清诊断中的应用比较。

摘要土拉菌病是一种由革兰氏阴性菌土拉菌引起的高度传染性人畜共患疾病。土拉菌病的微生物学诊断是基于细菌学、分子学和血清学调查。本文比较了市售酶联免疫吸附试验(ELISA)、自制酶联免疫吸附试验(ELISA)和试管凝集试验在兔热病血清诊断中的应用价值。方法:对57例有临床症状的兔热病患者、13例耶尔森菌病患者和20例献血者进行血清检测。国产ELISA检测IgA、IgG、IgM血清抗体的截止限为健康献血者血清抗体滴度平均值超过3个标准差。试管凝集试验阳性的临界值为滴度25。采用病毒粒子/系列ELISA法检测土拉菌脂多糖IgG和IgM抗体，并按生产厂家说明解释结果。结果:39例(68.4%)兔热病疑似患者血清中IgG和IgM抗体经试管凝集试验和病毒粒子/系列ELISA检测呈阳性。国产ELISA检测IgA/IgG抗体42份(73.7%)，IgM抗体39份(68.4%)。用试管凝集试验和自制ELISA检测耶尔森菌病患者和献血者血清均未见阳性反应。病毒粒子/系列ELISA仅在一名献血者中检测到具有诊断意义的IgG抗体。结论:三种血清学检测方法均可成功用于兔热病的常规血清学诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medycyna doswiadczalna i mikrobiologia

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