Gene expression profiling of gastric mucosa in mice lacking CCK and gastrin receptors

Chun-Mei Zhao , Yosuke Kodama , Arnar Flatberg , Vidar Beisvag , Bård Kulseng , Arne K. Sandvik , Jens F. Rehfeld , Duan Chen
{"title":"Gene expression profiling of gastric mucosa in mice lacking CCK and gastrin receptors","authors":"Chun-Mei Zhao ,&nbsp;Yosuke Kodama ,&nbsp;Arnar Flatberg ,&nbsp;Vidar Beisvag ,&nbsp;Bård Kulseng ,&nbsp;Arne K. Sandvik ,&nbsp;Jens F. Rehfeld ,&nbsp;Duan Chen","doi":"10.1016/j.regpep.2014.08.002","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>The stomach produces acid, which may play an important role in the regulation of bone homeostasis<span>. The aim of this study was to reveal signaling pathways<span> in the gastric mucosa that involve the </span></span></span>acid secretion and possibly the bone metabolism in CCK</span><sub>1</sub> and/or CCK<sub>2</sub><span> receptor knockout (KO) mice. Gastric acid secretion<span> was impaired and the ECL cell signaling pathway was inhibited in CCK</span></span><sub>2</sub> receptor KO mice but not in CCK<sub>1</sub> receptor KO mice. However, in CCK<sub>1<!--> <!-->+<!--> <!-->2</sub><span> receptor double KO mice the acid secretion in response to pylorus ligation-induced vagal stimulation and the ECL cell pathway were partially normalized, which was associated with an up-regulated pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1). The basal part of the gastric mucosa expressed parathyroid hormone-like hormone (PTHLH) in a subpopulation of likely ECL cells (and possibly other cells) and vitamin D3<span> 1α hydroxylase<span><span> probably in trefoil peptide2-immunoreactive cells. In conclusion, mice lacking CCK receptors exhibited a functional shift from the gastrin-CCK pathways to the neuronal pathway in control of the ECL cells and eventually the acid secretion. Taking the present data together with previous findings, we suggest a possible link between gastric PTHLH and </span>vitamin D and bone metabolism.</span></span></span></p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.08.002","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regulatory Peptides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167011514000627","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6

Abstract

The stomach produces acid, which may play an important role in the regulation of bone homeostasis. The aim of this study was to reveal signaling pathways in the gastric mucosa that involve the acid secretion and possibly the bone metabolism in CCK1 and/or CCK2 receptor knockout (KO) mice. Gastric acid secretion was impaired and the ECL cell signaling pathway was inhibited in CCK2 receptor KO mice but not in CCK1 receptor KO mice. However, in CCK1 + 2 receptor double KO mice the acid secretion in response to pylorus ligation-induced vagal stimulation and the ECL cell pathway were partially normalized, which was associated with an up-regulated pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1). The basal part of the gastric mucosa expressed parathyroid hormone-like hormone (PTHLH) in a subpopulation of likely ECL cells (and possibly other cells) and vitamin D3 1α hydroxylase probably in trefoil peptide2-immunoreactive cells. In conclusion, mice lacking CCK receptors exhibited a functional shift from the gastrin-CCK pathways to the neuronal pathway in control of the ECL cells and eventually the acid secretion. Taking the present data together with previous findings, we suggest a possible link between gastric PTHLH and vitamin D and bone metabolism.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
缺乏CCK和胃泌素受体小鼠胃粘膜的基因表达谱
胃产生酸,它可能在调节骨稳态中起重要作用。本研究的目的是揭示CCK1和/或CCK2受体敲除(KO)小鼠胃粘膜中涉及酸分泌和可能的骨代谢的信号通路。CCK2受体KO小鼠胃酸分泌受损,ECL细胞信号通路受到抑制,但CCK1受体KO小鼠没有受到抑制。然而,在CCK1 + 2受体双KO小鼠中,幽门结锁诱导的迷走神经刺激和ECL细胞通路的酸分泌部分正常化,这与垂体腺苷酸环化酶激活多肽(PACAP) 1型受体(PAC1)的上调有关。胃粘膜基底部在可能的ECL细胞亚群(也可能是其他细胞)中表达甲状旁腺激素样激素(PTHLH),在三叶肽2免疫反应细胞中表达维生素D3 1α羟化酶。综上所述,缺乏CCK受体的小鼠在控制ECL细胞和最终的酸分泌方面表现出从胃泌素-CCK途径到神经元途径的功能转变。将目前的数据与先前的发现结合起来,我们认为胃PTHLH与维生素D和骨代谢之间可能存在联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Regulatory Peptides
Regulatory Peptides 医学-内分泌学与代谢
自引率
0.00%
发文量
0
审稿时长
2 months
期刊介绍: Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.
期刊最新文献
WITHDRAWN: Effects of centrally-injected glucagon-like peptide-2 on gastric mucosal blood flow in rats; possible mechanisms. Editorial Board The neuro-incretin concept GLP-2: What do we know? What are we going to discover? Analgesic and anti-inflammatory effectiveness of sitagliptin and vildagliptin in mice
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1