Characterization of conformational states of POPC and DPPCd62 in POPC/DPPCd62/cholesterol mixtures using Raman spectroscopy

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry and Physics of Lipids Pub Date : 2023-08-12 DOI:10.1016/j.chemphyslip.2023.105337

Yu.V. Zaytseva, I.V. Zaytseva, N.V. Surovtsev

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Abstract

Conformational states of phospholipid chains in ternary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), deuterated 1,2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (DPPC_d62), and cholesterol (Chol) were studied by Raman spectroscopy. Parameters of Raman peaks sensitive to conformational order have been used to determine chain order for mixtures over a wide range of compositions. A ternary diagram of fractions of phospholipid chains in conformationally ordered and disordered states has been constructed. It was found that the addition of POPC and cholesterol increases the fraction of DPPC chains in disordered conformations. The so-called liquid-ordered phase includes DPPC molecules in both ordered and disordered states in comparable proportions. It was found that POPC chains are partially ordered in mixtures with DPPC and cholesterol, in contrast to the case of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). This maybe the underlying reason why ternary mixtures with POPC have different miscibility behavior compared to DOPC.

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用拉曼光谱表征POPC/DPPCd62/胆固醇混合物中POPC和DPPCd62的构象态

用拉曼光谱研究了1-棕榈酰-2-油基- n-甘油-3-磷酸胆碱(POPC)、氘化1,2-二棕榈酰-d62- n-甘油-3-磷酸胆碱(DPPCd62)和胆固醇(Chol)三元混合物中磷脂链的构象状态。对构象顺序敏感的拉曼峰参数已被用于确定大范围混合物的链序。构造了一种构象有序和无序状态的磷脂链的三元图。结果发现，POPC和胆固醇的加入增加了DPPC无序构象链的比例。所谓的液体有序相包括有序和无序状态的DPPC分子，其比例相当。结果表明，在DPPC和胆固醇的混合物中，POPC链是部分有序的，而在1,2-二甘油酯- n-甘油-3-磷脂胆碱(DOPC)中则相反。这可能是与DOPC相比，POPC三元混合物具有不同混相行为的根本原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.