Influenza A H1N1pdm 2009 Virus in Paraguay: Nucleotide Point Mutations in Hemagglutinin and Neuraminidase Genes are not Associated with Drug Resistance.

The Open Virology Journal Pub Date : 2014-09-29 eCollection Date: 2014-01-01 DOI:10.2174/1874357901408010009

Emilio E Espínola, Alberto A Amarilla, Magaly Martínez, Víctor H Aquino, Graciela Russomando

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引用次数: 3

Abstract

Influenza virus is associated with upper respiratory tract infections. The fourth influenza pandemic was declared in 2009. The aim of this study was to determine the genetic variability of the 2009 H1N1 pandemic virus circulating in Paraguay. Nasal swabs were collected from 181 patients with flu symptoms managed at the Hospital of the Medical School in Asunción, Paraguay, between August and October 2009. Virus detection was carried out by real-time reverse transcription-polymerase chain reaction, followed by sequencing of the hemagglutinin and neuraminidase genes, and phylogenetic analysis. H1N1pdm09 was detected in 14.9% (27/181) of the suspected cases. Analysis of 13 samples showed that these viruses the clustered in a single genetic group. Neither the mutation related to exacerbation of disease (D239G in hemagglutinin) nor that related to antiviral resistance (H275Y in neuraminidase), both detected in neighboring countries, were found. This genetic analysis of H1N1pdm09 will help to understand the spread of the disease.

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巴拉圭甲型H1N1pdm 2009病毒:血凝素和神经氨酸酶基因的核苷酸点突变与耐药性无关

流感病毒与上呼吸道感染有关。2009年宣布了第四次流感大流行。本研究的目的是确定在巴拉圭流行的2009年H1N1大流行病毒的遗传变异。2009年8月至10月期间，在巴拉圭Asunción医学院医院对181名有流感症状的患者进行了鼻拭子采集。采用实时逆转录聚合酶链反应进行病毒检测，随后进行血凝素和神经氨酸酶基因测序，并进行系统发育分析。14.9%(27/181)疑似病例检出H1N1pdm09。对13个样本的分析表明，这些病毒聚集在一个遗传群体中。没有发现与疾病恶化相关的突变(血凝素中的D239G)和与抗病毒药物耐药性相关的突变(神经氨酸酶中的H275Y)，这两种突变均在邻国检测到。对H1N1pdm09的基因分析将有助于了解这种疾病的传播。

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The Open Virology Journal

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